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A Micro Biuret Method for Protein Determination Determination of Total Protein in Cerebrospinal Fluid
Abstract
A rapid and convenient colorimetric method for the determination of small amounts of protein, based on the biuret reaction and readings at 330 mμ, is described. The “micromethod” allows the determination of 10 to 200 mg per cent protein with an acurassy of ± 1.5 mg per cent. An “ultra micro” modification allows determinations of 10 to 400 micrograms protein.The method is applied for the determination of total protein in 1 ml of cerebrospinal fluid.
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Citations
Cited by
Brandtzaeg, P., Fjellanger, I., & Gjeruldsen, S. T. (1970) · Scandinavian Journal of Haematology · doi:10.1111/j.1600-0609.1970.tb01956.x · Cited by 314
Moghimi, S. M. (2002) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/s0167-4889(02)00204-5 · Cited by 103
Metadata
- Authors
- Goa, J.
- Venue
- Scandinavian Journal of Clinical and Laboratory Investigation
- Publisher
- Informa UK Limited
- Published
- 1953-01
- Volume
- 5
- Issue
- 3
- Pages
- 218-222
- ISSN
- 0036-5513 (ISSN-L)
- 1502-7686
- OpenAlex
- W2079938883
- Cited by
- 1,086
- References
- 0
- FWCI
- 2.24
- Type
- journal article
- Language
- en
- Open access
- closed
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- Goa, J. (1953). A Micro Biuret Method for Protein Determination Determination of Total Protein in Cerebrospinal Fluid. Scandinavian Journal of Clinical and Laboratory Investigation. https://doi.org/10.3109/00365515309094189
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.