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The Use of Fluorescein Diacetate and Phenosafranine for Determining Viability of Cultured Plant Cells
Abstract
Various stains were tested with suspension cell cultures of Nicotiana tabacum (tobacco), Oryza sativa (rice), Glycine max (soybean), Daucus carota (carrot) and Lycopersicon esculentum (tomato). A drop of the culture medium containing each dye was mixed with a drop of cells on a microscope slide and viewed after 5 min. The most suitable dyes tested were fluorescein diacetate for viable cells and phenosafranine for dead cells. These dyes stained specifically whether the cells were from cultures of different ages, of different species or if the cells had been treated in various, often toxic, ways. The viability of the cells was confirmed by observing cyclosis and plasmolysis, and whether the cells could grow further when cultured.
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Citations
Cited by
Jacyn Baker, C., & Mock, N. M. (1994) · Plant Cell, Tissue and Organ Culture · doi:10.1007/bf00037585 · Cited by 600
Harvesting Chlorella minutissima using cell coagulants
Papazi, A., Makridis, P., & Divanach, P. (2010) · Journal of Applied Phycology · doi:10.1007/s10811-009-9465-2 · Cited by 301
Filonova, L. H., Bozhkov, P. V., Brukhin, V. B., Daniel, G., Zhivotovsky, B., & Arnold, S. V. (2000) · Journal of Cell Science · doi:10.1242/jcs.113.24.4399 · Cited by 257
Initiation, growth and cryopreservation of plant cell suspension cultures
Mustafa, N. R., de Winter, W., van Iren, F., & Verpoorte, R. (2011) · Nature Protocols · doi:10.1038/nprot.2010.144 · Cited by 235
Elicitation studies in cell suspension cultures of Cannabis sativa L.
Flores-Sanchez, I. J., Peč, J., Fei, J., Choi, Y. H., Dušek, J., & Verpoorte, R. (2009) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2009.05.006 · Cited by 81
Metadata
- Authors
- Widholm, J. M.
- Venue
- Stain Technology
- Publisher
- Informa UK Limited
- Published
- 1972-01
- Volume
- 47
- Issue
- 4
- Pages
- 189-194
- ISSN
- 0038-9153 (ISSN-L)
- OpenAlex
- W2026311140
- Cited by
- 1,413
- References
- 0
- FWCI
- 1.56
- Type
- journal article
- Language
- en
- Open access
- closed
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- Widholm, J. M. (1972). The Use of Fluorescein Diacetate and Phenosafranine for Determining Viability of Cultured Plant Cells. Stain Technology. https://doi.org/10.3109/10520297209116483
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
