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Enzyme Clearing of Alcian Blue Stained Whole Small Vertebrates for Demonstration of Cartilage
Abstract
Preparation of small vertebrates cleared after alcian blue staining of cartilage is facilitated by trypsin digestion. Specimens are fixed in formation, washed, skinned, and eviscerated. After staining in a solution of alcian blue in acetic acid-alcohol for 24-48 hours, they are transferred to water through graded alcohols. Excess alcian blue is removed over a period of up to three weeks by changes every 2-3 days of 1% trypsin in approximately one-third-saturated sodium borate. Bony tissues may be stained after this in a solution of alizarin red S in 0.5% KOH. Specimens are bleached if necessary and dehydrated through graded KOH-glycerine mixtures for storage in glycerine. Since alcohol treatment in addition to formalin fixation does not affect results with this method, it should be useful to researchers who want to study the cartilage or cartilaginous skeletons in museum specimens, which are routinely fixed in formalin and stored in alcohol.
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Cited by
Poux, C., Madsen, O., Marquard, E., Vieites, D. R., de Jong, W. W., & Vences, M. (2005) · Systematic Biology · doi:10.1080/10635150500234534 · Cited by 339
Micro‐anatomy of the pectoral fin in blennies (Blenniini, Blennioidea, Teleostei)
Brandstätter, R., Misof, B., Pazmandi, C., & Wagner, G. P. (1990) · Journal of Fish Biology · doi:10.1111/j.1095-8649.1990.tb02537.x · Cited by 28
Jude, E., Johanson, Z., Kearsley, A., & Friedman, M. (2014) · Frontiers in Earth Science · doi:10.3389/feart.2014.00018 · Cited by 15
Aiello, B. R., Hardy, A. R., Cherian, C., Olsen, A. M., Orsbon, C. P., Hale, M. E., & Westneat, M. W. (2018) · Journal of Morphology · doi:10.1002/jmor.20830 · Cited by 14
Metadata
- Authors
- Dingerkus, G., & Uhler, L. D.
- Venue
- Stain Technology
- Publisher
- Informa UK Limited
- Published
- 1977-01
- Volume
- 52
- Issue
- 4
- Pages
- 229-232
- ISSN
- 0038-9153 (ISSN-L)
- OpenAlex
- W2008668441
- Cited by
- 1,285
- References
- 0
- FWCI
- 3.36
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Enzyme Clearing of Alcian Blue Stained Whole Small Vertebrates for Demonstration of Cartilage. https://app.cochat.ai/papers/10.3109/10520297709116780
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- Dingerkus, G., & Uhler, L. D. (1977). Enzyme Clearing of Alcian Blue Stained Whole Small Vertebrates for Demonstration of Cartilage. Stain Technology. https://doi.org/10.3109/10520297709116780
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
