The phenylthiourea is a competitive inhibitor of the enzymatic oxidation of DOPA by phenoloxidase
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Phenoloxidase is a key enzyme of melanization catalyzing the oxidation of phenols. Phenylthiourea (PTU) is the well-known and widely used inhibitor of phenoloxidase. However, the mechanism of its action is not quite clear. In the present work, the effect of PTU on the enzymatic oxidation of 3-(3,4-dihydroxyphenyl)-l-alanine (DOPA) by phenoloxidase was studied by spectrophotometric methods. The inhibition constant of PTU was estimated as 0.21 ± 0.09 µM and the competitive type of inhibition was determined for this reaction.
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PTU has been shown to interfere not only with tyrosinase but also with other enzymes, such as thyroid peroxidase and dopamine beta-hydroxylase.
“For example, PTU has been shown to interfere not only with tyrosinase (the enzyme that catalyses the production of melanin)1011, but also with other enzymes, such as thyroid peroxidase12 and dopamine beta-hydroxylase13.”
From A crystal-clear zebrafish for in vivo imaging (2016) · cites this paper as [11]
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A crystal-clear zebrafish for in vivo imaging
Antinucci, P., & Hindges, R. (2016) · Scientific Reports · doi:10.1038/srep29490 · Cited by 165 · see citing works
- Authors
- Ryazanova, A. D., Alekseev, A. A., & Slepneva, I. A.
- Affiliations
- Institute of Chemical Kinetics and Combustion (RU)
- Corresponding author
- Ryazanova, Alexandra D.
- Venue
- Journal of Enzyme Inhibition and Medicinal Chemistry
- Publisher
- Informa UK Limited
- Published
- 2011-05-03
- Volume
- 27
- Issue
- 1
- Pages
- 78-83
- ISSN
- 1475-6366 (ISSN-L)
- 1475-6374
- OpenAlex
- W2083406213
- Cited by
- 69
- References
- 0
- Citations by year
- 2026: 5 · 2025: 6 · 2024: 5 · 2023: 8 · 2022: 7 · 2021: 5 · 2020: 6 · 2019: 3 · 2018: 2 · 2017: 5 · 2016: 3 · 2015: 6 · 2014: 4 · 2013: 1 · 2012: 3
- FWCI
- 1.15
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Enzyme Inhibition and Medicinal Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme function and inhibition
- Keywords
- phenylthiourea, phenoloxidase, DOPA oxidation, competitive inhibition, enzyme inhibition, melanization, inhibition constant, enzyme kinetics
- MeSH terms
- Dihydroxyphenylalanine, Dose-Response Relationship, Drug, Enzyme Inhibitors, Oxidation-Reduction, Phenylthiourea, Structure-Activity Relationship, Monophenol Monooxygenase, Biocatalysis
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