Biomedical nanoparticles modulate specific CD4+T cell stimulation by inhibition of antigen processing in dendritic cells
Open accessAbstract
Understanding how nanoparticles may affect immune responses is an essential prerequisite to developing novel clinical applications. To investigate nanoparticle-dependent outcomes on immune responses, dendritic cells (DCs) were treated with model biomedical poly(vinylalcohol)-coated super-paramagnetic iron oxide nanoparticles (PVA-SPIONs). PVA-SPIONs uptake by human monocyte-derived DCs (MDDCs) was analyzed by flow cytometry (FACS) and advanced imaging techniques. Viability, activation, function, and stimulatory capacity of MDDCs were assessed by FACS and an in vitro CD4+ T cell assay. PVA-SPION uptake was dose-dependent, decreased by lipopolysaccharide (LPS)-induced MDDC maturation at higher particle concentrations, and was inhibited by cytochalasin D pre-treatment. PVA-SPIONs did not alter surface marker expression (CD80, CD83, CD86, myeloid/plasmacytoid DC markers) or antigen-uptake, but decreased the capacity of MDDCs to process antigen, stimulate CD4+ T cells, and induce cytokines. The decreased antigen processing and CD4+ T cell stimulation capability of MDDCs following PVA-SPION treatment suggests that MDDCs may revert to a more functionally immature state following particle exposure.
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Activation of dendritic cells has been observed upon exposure to poly(vinylalcohol)-coated SPION.
“Additionally, activation of dendritic cells has been observed upon exposure to poly(vinylalcohol)-coated SPION [125].”
From Iron oxide nanoparticles: Diagnostic, therapeutic and theranostic applications (2019) · cites this paper as [125]
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References 56 · Cited by 97
- Authors
- Blank, F., Gerber, P., Rothen-Rutishauser, B., Sakulkhu, U., Salaklang, J., De Peyer, K., Gehr, P., Nicod, L. P., Hofmann, H., Geiser, T., Petri-Fink, A., & Von Garnier, C.
- Affiliations
- University Hospital of Bern (CH)
- École Polytechnique Fédérale de Lausanne (CH)
- University of Fribourg (CH)
- University of Bern (CH)
- Centre Hospitalier Universitaire Vaudois (CH)
- Venue
- Nanotoxicology
- Publisher
- Informa UK Limited
- Published
- 2011-01-13
- Volume
- 5
- Issue
- 4
- Pages
- 606-621
- ISSN
- 1743-5390 (ISSN-L)
- 1743-5404
- OpenAlex
- W2105985995
- Cited by
- 97
- References
- 56
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 4 · 2023: 3 · 2022: 4 · 2021: 9 · 2020: 10 · 2019: 6 · 2018: 5 · 2017: 9 · 2016: 12 · 2015: 5 · 2014: 11 · 2013: 5 · 2012: 4
- FWCI
- 3.25
- Type
- journal article
- Language
- en
- Open access
- green
- infoscience.epfl.ch
- Locations
- Nanotoxicology (publishedVersion)
- PubMed (publishedVersion)
- reroDoc Digital Library (submittedVersion)
- Bern Open Repository and Information System (University of Bern) (submittedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- IRIS (submittedVersion)
- SERVAL (Université de Lausanne) (submittedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- biomedical nanoparticles, superparamagnetic iron oxide nanoparticles, polyvinyl alcohol coating, dendritic cells, monocyte-derived dendritic cells, antigen processing, CD4+ T cell stimulation, nanoparticle uptake, dendritic cell maturation, lipopolysaccharide, cytochalasin D
- MeSH terms
- Cells, Cultured, Dendritic Cells, Ferric Compounds, Flow Cytometry, HLA-D Antigens, Humans, Lipopolysaccharides, CD4-Positive T-Lymphocytes, Cytochalasin D, Apoptosis, Antigen Presentation, Magnetite Nanoparticles
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