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Chymosin-Mediated Proteolysis, Calcium Solubilization, and Texture Development During the Ripening of Cheddar Cheese
Open accessAbstract
Full fat, milled-curd Cheddar cheeses (2 kg) were manufactured with 0.0 (control), 0.1, 1.0, or 10.0 micromol of pepstatin (a potent competitive inhibitor of chymosin) added per liter of curds/whey mixture at the start of cooking to obtain residual chymosin levels that were 100, 89, 55, and 16% of the activity in the control cheese, respectively. The cheeses were ripened at 8 degrees C for 180 d. There were no significant differences in the pH values of the cheeses; however, the moisture content of the cheeses decreased with increasing level of pepstatin addition. The levels of pH 4.6-soluble nitrogen in the 3 cheeses with added pepstatin were significantly lower than that of the control cheese at 1 d and throughout ripening. Densitometric analysis of urea-PAGE electro-phoretograms of the pH 4.6-insoluble fractions of the cheese made with 10.0 micromol/L of pepstatin showed complete inhibition of hydrolysis of alpha(S1)-casein (CN) at Phe23-Phe24 at all stages of ripening. The level of insoluble calcium in each of 4 cheeses decreased significantly during the first 21 d of ripening, irrespective of the level of pepstatin addition. Concurrently, there was a significant reduction in hardness in each of the 4 cheeses during the first 21 d of ripening. The softening of texture was more highly correlated with the level of insoluble calcium than with the level of intact alpha(S1)-CN in each of the 4 cheeses early in ripening. It is concluded that hydrolysis of alpha(S1)-CN at Phe23-Phe24 is not a prerequisite for softening of Cheddar cheese during the early stages of ripening. We propose that this softening of texture is principally due to the partial solubilization of colloidal calcium phosphate associated with the para-CN matrix of the curd.
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Metadata
- Authors
- O’Mahony, J. A., Lucey, J. A., & McSweeney, P. L. H.
- Affiliations
- University of Wisconsin–Madison (US)
- University College Cork (IE)
- Corresponding author
- McSweeney, P.L.H.
- Venue
- Journal of Dairy Science
- Publisher
- American Dairy Science Association
- Published
- 2005-09
- Volume
- 88
- Issue
- 9
- Pages
- 3101-3114
- ISSN
- 0022-0302 (ISSN-L)
- OpenAlex
- W2141054859
- Cited by
- 160
- References
- 65
- Citations by year
- 2026: 5 · 2025: 10 · 2024: 10 · 2023: 5 · 2022: 13 · 2021: 7 · 2020: 5 · 2019: 11 · 2018: 5 · 2017: 13 · 2016: 4 · 2015: 14 · 2014: 9 · 2013: 7 · 2012: 4
- FWCI
- 10.02
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Dairy Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National University of Ireland
- University of Wisconsin-Madison
- Dairy Management Inc
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
- Life Sciences › Agricultural and Biological Sciences › Food Science › Advanced Scientific Research Methods
- Keywords
- Cheddar cheese, cheese ripening, chymosin, pepstatin, calcium phosphate solubilization, hardness, proteolysis, moisture content
- MeSH terms
- Calcium, Calcium Phosphates, Caseins, Cheese, Colloids, Food Handling, Food Technology, Hydrogen-Ion Concentration, Hydrolysis, Pepstatins, Chymosin, Solubility
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