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Superiority of Fluorescein Isothiocyanate (Riggs) for Fluorescent-Antibody Technic with a Modification of its Application
Abstract
1) The globulin fractions of antisera representing bacterial, viral, mycotic agents and antiglobulin fractions were labeled with 2 derivatives of fluorescein amine by 3 methods. 2) Fluorescein isothiocyanate was shown to be superior in stability, ease of conjugation, and degree of fluorescence. This direct method of adding the dye to a dilute buffered antiserum eliminates the need for organic reagents which may denature the protein.
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Cited by
Isolation of the Etiologic Agent of Korean Hemorrhagic Fever
Lee, H. W., Lee, P. W., & Johnson, K. M. (2004) · Journal of Infectious Diseases · doi:10.1093/infdis/190.9.1711 · Cited by 800
Immunofluorescence and Herpes-Type Virus Particles in the P3HR-1 Burkitt Lymphoma Cell Line
Hinuma, Y., Konn, M., Yamaguchi, J., Wudarski, D. J., Blakeslee, J. R., & Grace, J. T. (1967) · Journal of Virology · doi:10.1128/jvi.1.5.1045-1051.1967 · Cited by 493
BRANDTZAEG, P. (1973) · Scandinavian Journal of Immunology · doi:10.1111/j.1365-3083.1973.tb02037.x · Cited by 289
Ooi, B. S., Jao, W., First, M. R., Mancilla, R., & Pollak, V. E. (1975) · The American Journal of Medicine · doi:10.1016/0002-9343(75)90223-5 · Cited by 102
Metadata
- Authors
- Marshall, J. D., Eveland, W. C., & Smith, C. W.
- Venue
- Experimental Biology and Medicine
- Publisher
- Frontiers Media SA
- Published
- 1958-08-01
- Volume
- 98
- Issue
- 4
- Pages
- 898-900
- ISSN
- 0071-3384 (ISSN-L)
- 1535-3702
- 1535-3699
- OpenAlex
- W2315085743
- Cited by
- 412
- References
- 0
- FWCI
- 52.51
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Superiority of Fluorescein Isothiocyanate (Riggs) for Fluorescent-Antibody Technic with a Modification of its Application. https://app.cochat.ai/papers/10.3181/00379727-98-24222
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- Marshall, J. D., Eveland, W. C., & Smith, C. W. (1958). Superiority of Fluorescein Isothiocyanate (Riggs) for Fluorescent-Antibody Technic with a Modification of its Application. Experimental Biology and Medicine. https://doi.org/10.3181/00379727-98-24222
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
