Comparison and evaluation of different methodologies and tests for detection of anti-dsDNA antibodies on 889 Slovenian patients’ and blood donors’ sera

Žigon, Polona; Lakota, Katja; Čučnik, Saša; Švec, Tinka; Ambrožič, Aleš; Sodin-Šemrl, Snežna; Kveder, Tanja

doi:10.3325/cmj.2011.52.694

Comparison and evaluation of different methodologies and tests for detection of anti-dsDNA antibodies on 889 Slovenian patients’ and blood donors’ sera

Aim To evaluate four different commercially available assays for anti-double stranded DNA (dsDNA) detection and compare them with the in-house radioimmunoassay according to Farr (FARR-RIA) in order to select the optimal primary method for use in combination with FARR-RIA. Methods Sera from 583 co…