The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Application of gene probes to detect a penaeid shrimp baculovirus in fixed tissue using in situ hybridization
Open accessAbstract
DNA inserts obtained from a cloning of the penae~d shrimp baculovirus, BP, were nonradioactively labelled with digoxigenin for use as gene probes to detect BP in paraffin-embedded, formalin/alcohol-fixed tissue sections using in sit11 hybridization.Results show that the probes detect BP in various species of shrimp from different geoglaphicdl dreas.The probes demonstrate no reaction to BP-negative tissue sections.The probes also demonstrate no reaction to tissue infected with other penaeid shrimp viruses.
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“Neutral-buffered formalin, Z-fix®, and SafeFix® II are fixatives of choice for molecular applications, such as in situ hybridizations (e.g., Bruce et al. , 1993; Carnegie et al. , 2003; Li et al. , 2006; Small et al. , 2007)”
From Collection techniques for the analyses of pathogens in crustaceans (2017) · cites this paper as Bruce et al. , 1993
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Cites
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Metadata
- Authors
- Bruce, L., Redman, R., Lightner, D., & Bonami, Jr.
- Affiliations
- University of Arizona (US)
- Venue
- Diseases of Aquatic Organisms
- Publisher
- Inter-Research Science Center
- Published
- 1993
- Volume
- 17
- Pages
- 215-221
- ISSN
- 0177-5103 (ISSN-L)
- 1616-1580
- OpenAlex
- W1979582118
- Cited by
- 34
- References
- 3
- Citations by year
- 2023: 1 · 2017: 2
- FWCI
- 1.66
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Diseases of Aquatic Organisms (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
- Physical Sciences › Environmental Science › Ecology › Crustacean biology and ecology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Keywords
- gene probes, in situ hybridization, paraffin-embedded tissue, shrimp viruses, virus detection
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