The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Experimental vertical transmission of Piscirickettsia salmonis and in vitro study of attachment and mode of entrance into the fish ovum
Open accessAbstract
Piscirickettsia salmonis is a pathogenic bacterial agent causing septicaemic disease in salmon. Since its isolation in Chile in 1989, P. salmonis has continually produced high mortality rates in salmon farms. Little information exists regarding the mechanisms of vertical transmission of this pathogen. Experimental vertical transmission was established in the present study by inoculation of male and female rainbow trout broodstock with P. salmonis. The bacterium was subsequently detected using indirect immunofluorescence in milt and coelomic fluid of the majority of inoculated broodstock (14/15). Bacteria were detected in the fry when 1 or both parents were inoculated, although none of the infected fry presented signs of the disease. P. salmonis was also detected in progeny obtained through fertilisation ova from non-inoculated females incubated in a medium containing a bacterial suspension, demonstrating transmission during the process of fertilisation. Ova infected in vitro were examined at sample periods from 30 s to 60 min using scanning electron microscopy. This demonstrated that the bacterium attaches to the ova by means of membrane extensions, structures which we have called 'piscirickettsial attachment complex' (PAC) and which would allow later penetration into the ovum.
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“Vertical transmission of bacteria has also been suggested in marine organisms such as fish and sponges and has been experimentally proved in salmons (Larenas et al., 2003; Lee et al., 2009; Ikeda-Ohtsubo et al., 2018; Nyholm, 2020).”
From The Active Microbiota of the Eggs and the Nauplii of the Pacific Blue Shrimp Litopenaeus stylirostris Partially Shaped by a Potential Vertical Transmission (2022) · cites this paper as Larenas et al., 2003
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Giraud, C., Callac, N., Boulo, V., Lam, J.-S., Pham, D., Selmaoui-Folcher, N., & Wabete, N. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.886752 · Cited by 26
Metadata
- Authors
- Larenas, J., Bartholomew, J., Troncoso, O., Fernández, S., Ledezma, H., Sandoval, N., Vera, P., Contreras, J., & Smith, P.
- Affiliations
- University of Chile (CL)
- Oregon State University (US)
- Corresponding author
- Larenas, JJ
- Venue
- Diseases of Aquatic Organisms
- Publisher
- Inter-Research Science Center
- Published
- 2003
- Volume
- 56
- Issue
- 1
- Pages
- 25-30
- ISSN
- 0177-5103 (ISSN-L)
- 1616-1580
- OpenAlex
- W2008634047
- Cited by
- 43
- References
- 19
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 5 · 2021: 3 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 3 · 2015: 2 · 2014: 6 · 2013: 5 · 2012: 2
- FWCI
- 0.43
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Diseases of Aquatic Organisms (publishedVersion)
- PubMed (publishedVersion)
- Americanae (AECID Library) (submittedVersion, other-oa)
- Americanae (AECID Library) (submittedVersion, cc-by-nc-nd)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by-nc-nd)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
- Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
- Life Sciences › Immunology and Microbiology › Parasitology › Parasite Biology and Host Interactions
- Keywords
- Piscirickettsia salmonis, vertical transmission, rainbow trout, fertilisation (biology), ova
- MeSH terms
- Animals, Bacterial Infections, Chile, Female, Fish Diseases, Fluorescent Antibody Technique, Male, Microscopy, Electron, Scanning, Ovum, Oncorhynchus mykiss, Infectious Disease Transmission, Vertical, Gammaproteobacteria, In Vitro Techniques
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