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Cited by 81Protist diversity and phylogenyMicrobial Community Ecology and PhysiologyMarine Plankton and Biogeochemistry

The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.

Effect of fixation on particle retention by microflagellates: underestimation of grazing rates

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Sieracki, ME, Haas, LW, Caron, DA, Lessard, EJ·1987·Marine Ecology Progress Series
DOI 10.3354/meps038251
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Abstract

The uptake of fluorescent particles by protists and filter-feeding metazoa is being used increasingly by microbial ecologists to study feeding behavior and measure grazing rates.Recent studies of microflagellate uptake of these inert particles have yielded inconsistent results.In particular.grazing rates determined from fluorescent particle uptake are often less than rates measured using other techniques.These low uptake rates have been attributed to osmotrophy, food quality or size selection, rapid egestion of inert particles, and the slower feeding by free-living, as opposed to attached, protists.We have found that a variety of flagellates egest food vacuole contents upon fixation with several commonly used agents including glutaraldehyde and formaldehyde.During time course experiments, the observed microsphere uptake rate for a small chrysomonad flagellate using 1 % glutaraldehyde was only 6 % of the rate obtained by using the fixation method of van der Veer (1982) (2 '10 acrolein, 2 % glutaraldehyde and 1 % tannic acid), modified for epifluorescence microscopy.Uptake rates of several mixed flagellate populations also were 2.4 to 3.1 times higher using the modified van der Veer method than with 1 % glutaraldehyde.The average number of ingested microspheres cell-' using this method was simllar to that observed in live cells immobilized with NiS04.Glutaraldehyde also caused the egestion of Synechococcus sp. cells and fluorescently labelled bacteria from the chrysomonad flagellate.We conclude that previous studies using common aldehyde fixation wlth particle uptake for measunng rates of microflagellate bacterivory have significantly underestimated actual rates of consumpt~on, and that these studes must be re-evaluated, and perhaps repeated, using effective fixation methods.

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Metadata

Authors
Sieracki, M., Haas, L., Caron, D., & Lessard, E.
Venue
Marine Ecology Progress Series
Publisher
Inter-Research Science Center
Published
1987
Volume
38
Pages
251-258
ISSN
  • 0171-8630 (ISSN-L)
  • 1616-1599
DOI
10.3354/meps038251
OpenAlex
W2048477826
Cited by
81
References
18
Citations by year
2022: 2 · 2019: 1 · 2017: 1 · 2016: 2 · 2012: 1
FWCI
2.96
Type
journal article
Language
en
Open access
Locations
  • Marine Ecology Progress Series (publishedVersion)
  • W&M Publish (College of William & Mary) (submittedVersion)
Funders
National Science Foundation (8316613, 8600510, 8617565)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Protist diversity and phylogeny
  • Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
  • Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine Plankton and Biogeochemistry
Keywords
microflagellates, particle retention, grazing rates, aldehyde fixation, glutaraldehyde, formaldehyde, egestion, chrysomonads, bacterivory, Synechococcus, epifluorescence microscopy

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