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Cited by 331

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Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC

Open access
Barlow, RG, Cummings, DG, Gibb, SW·1997·Marine Ecology Progress Series

Abstract

A reverse phase HPLC (hlgh performance liquid chromatography) technique using a binary solvent system with a linear gradient on a Hypersilm MOS2 C-8 column is described.As well as the resolution of key chemotaxonom~c chlorophyll and carotenoid pigments, baseline separation of mono-and divinyl chlorophyll a and of lutein and zeaxanthin.and partial separation of mono-and dlvinyl chlorophyll b are a c h ~e v e d in a total analysis time of less than 30 min.The method provides an optimal balance between analyte resolutlon and sample throughput and IS hence well suited to the analysis of oceanographic samples.

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Metadata

Authors
Barlow, R., Cummings, D., & Gibb, S.
Venue
Marine Ecology Progress Series
Published
1997
Volume
161
Pages
303-307
ISSN
  • 0171-8630 (ISSN-L)
  • 1616-1599
OpenAlex
W1996851933
Cited by
331
References
0
FWCI
3.58
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC. https://app.cochat.ai/papers/10.3354/meps161303
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Barlow, R., Cummings, D., & Gibb, S. (1997). Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC. Marine Ecology Progress Series. https://doi.org/10.3354/meps161303

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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.