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Cited by 373D Printing in Biomedical ResearchBone Tissue Engineering MaterialsTissue Engineering and Regenerative Medicine

Cell Seeding Process Experiment and Simulation on Three-Dimensional Polyhedron and Cross-Link Design Scaffolds

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Liu, Ziyu, Tamaddon, Maryam, Gu, Yingying, Yu, Jianshu, Xu, Nan, Gang, Fangli, Sun, Xiaodan, Liu, Chaozong·2020·Frontiers in Bioengineering and Biotechnology
DOI 10.3389/fbioe.2020.00104PMC PMC7064471
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Abstract

Controlled cell seeding on scaffolds is a significant step towards successful tissue engineering. It is essential to know the cell seeding efficiency and distribution to be able to optimize the design of tissue engineering scaffolds. Two type of scaffolds, including truncated octahedron structure and cubic structure, were designed for vitro cell seeding investigation and subsequently modification for simulation approach. Both of scaffold showed a gradual lateral variation of attached cells, and live cells movement are exremly slow by only diffusion while dead cell can not move without external force. A simulation approach, by ANSYS Fluent coupling the VOF model, DPM model and CIM model, was developed for cell seeding process in scaffold. The simulation approches supply a more accurate model to simulate cells adhesion for three-dimension structure. As the initial stages of cell attachment in vivo are hard to observe, this novel method provides an opportunity to predict cells distribution, thereby helping to optimize scaffold structures. As tissue formation is highcly related to the cell distribution, this model may help researchers to predict the effect of applied scaffold and reduce the number of animal testing.

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  • The traditional tissue engineering approach utilizes cell-seeded constructs, often loaded with exogenous growth factors or cytokines, to reconstruct damaged tissue.

    “The traditional TE approach utilizes cell-seeded constructs, often loaded with exogenous growth factors or cytokines, to reconstruct the damaged tissue [230].”

    From Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review (2023) · cites this paper as [230]

    The Introduction describes cell attachment to scaffolds in tissue engineering and gives an example in which a scaffold fills the void left by diseased tissue. Neither the Introduction nor the retrieved cell-seeding methods passages say that such constructs are often loaded with exogenous growth factors or cytokines. (LLM, conf=0.86)

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Cited by

Sorted by citations

Authors
Liu, Z., Tamaddon, M., Gu, Y., Yu, J., Xu, N., Gang, F., Sun, X., & Liu, C.
Affiliations
  • Royal National Orthopaedic Hospital (GB)
  • State Key Laboratory of New Ceramics and Fine Processing (CN)
Corresponding author
  • Sun, Xiaodan
  • Liu, Chaozong
ORCID
Venue
Frontiers in Bioengineering and Biotechnology
Publisher
Frontiers Media SA
Published
2020-03-04
Volume
8
Pages
104-104
ISSN
2296-4185 (ISSN-L)
DOI
10.3389/fbioe.2020.00104
PMC
PMC7064471
OpenAlex
W3009705587
Cited by
37
References
0
Citations by year
2026: 1 · 2025: 8 · 2024: 7 · 2023: 5 · 2022: 9 · 2021: 7
FWCI
1.65
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Bioengineering and Biotechnology (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • UCL Discovery (University College London) (submittedVersion)
Funders
Arthritis Research UK
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
cell seeding, tissue engineering scaffolds, truncated octahedron, scaffold design optimization, cell distribution, cell adhesion, cell diffusion, volume of fluid method, discrete particle model

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