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Cited by 45Biofuel production and bioconversionEnzyme Production and CharacterizationAnaerobic Digestion and Biogas Production

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Developing Efficient Thermophilic Cellulose Degrading Consortium for Glucose Production From Different Agro-Residues

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Singh, Saurabh, Jaiswal, Durgesh Kumar, Sivakumar, Nallusamy, Verma, Jay Prakash·2019·Frontiers in Energy Research
DOI 10.3389/fenrg.2019.00061
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Abstract

In the present study, eleven cellulose degrading bacterial strains were isolated from water and soil samples of hot springs in the Chumathang village, Leh and Ladakh region, India. The isolated strains were identified as Bacillus subtilis, Bacillus aryabhattai, Bacillus stratosphericus, Bacillus altitudinis and Brevibacterium frigoritolerans by biochemical and molecular approaches. All the strains were evaluated for the total cellulase, endoglucanase, exoglucanase, and β-glucosidase enzyme activities. On the basis of overall individual cellulose degrading enzyme activities, three strains were selected to develop consortium to enhance their cellulase enzyme activities. The potent cellulose degraders B. stratosphericus BHUJPV-H5, B. subtilis BHUJPV-H19 and B. subtilis BHUJPV-H12 were selected for the consortium development. The effect of cellulase activities of bacterial strains were evaluated ranged up to 6.06 and 0.72 mg ml-1glucose by agro-residues of sugarcane bagasse and wheat straw, respectively after 1 h of incubation. Total cellulase enzyme activity of consortium was recorded two times higher than the individual organisms. These strains can be used for enhancing bioethanol production from lignocellulosic biomass, which can consequently boost biofuel production industry.

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Metadata

Authors
Singh, S., Jaiswal, D. K., Sivakumar, N., & Verma, J. P.
Affiliations
  • Banaras Hindu University (IN)
  • Sultan Qaboos University (OM)
Corresponding author
Verma, Jay Prakash
ORCID
Venue
Frontiers in Energy Research
Publisher
Frontiers Media SA
Published
2019-07-02
Volume
7
ISSN
2296-598X (ISSN-L)
DOI
10.3389/fenrg.2019.00061
OpenAlex
W2955581643
Cited by
45
References
0
Citations by year
2026: 6 · 2025: 10 · 2024: 10 · 2023: 4 · 2022: 7 · 2021: 6 · 2020: 2
FWCI
1.32
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Energy Research (publishedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
Council of Scientific and Industrial Research, India
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Anaerobic Digestion and Biogas Production
Keywords
thermophilic bacteria, cellulolytic bacteria, bacterial consortium, glucose production, agro-residues, sugarcane bagasse, wheat straw, hot spring isolates, Bacillus stratosphericus, Bacillus subtilis, cellulase activity, endoglucanase, cellobiohydrolase, β-glucosidase, bioethanol production, lignocellulosic biomass, biofuels

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