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Cited by 14Identification and Quantification in FoodForensic and Genetic ResearchGenetic diversity and population structure

Development and Validation of a Novel Five-Dye Short Tandem Repeat Panel for Forensic Identification of 11 Species

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Cui, Wei, Jin, Xiaoye, Guo, Yuxin, Chen, Chong, Zhang, Wenqing, Wang, Yijie, Lan, Jiangwei, Zhu, Bofeng·2020·Frontiers in Genetics
DOI 10.3389/fgene.2020.01005PMC PMC7541953
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Abstract

Species identification of unknown biological samples is of fundamental importance for forensic applications, especially in crime detection, poaching and illegal trade of endangered animals as well as meat fraud. In this study, a novel panel was developed that can simultaneously identify 10 different animal species (Gallus gallus domesticus, Anas platyrhynchos domesticus, Ovis aries, Sus scrofa domesticus, Bos taurus, Equus caballus, Columba livia domestica, Rattus norvegicus, Mus musculus, Canis lupus familiaris) and human beings by amplifying 22 short tandem repeat (STR) loci in a multiplex PCR using a set of five fluorescently labeled dyes. This novel 22-STR panel was validated by optimization of PCR conditions as well as sensitivity, reproducibility, precision, species specificity, DNA mixture, and tissue/organ consistency. The results of developmental validation showed that the 22 STR loci achieved high species specificity among 10 animal species and human beings, and the sensitivity of this panel was 0.09 ng. This 22-STR panel identified different meat in mixed samples, and the minimum detected mixture ratio in the current test was 10% (0.1 ng/1 ng). This sensitive, accurate, and specific 22-STR panel can be used for forensic species identification and the detection of meat fraud and adulteration.

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  • Species-specific or custom-designed STR and SNP arrays to identify different breeds are becoming more common.

    “For non-human models, species-specific or custom-designed STR and SNP arrays to identify different breeds are becoming more common [62,63,64].”

    From Genetic Insights into the Economic Toll of Cell Line Misidentification: A Comprehensive Review (2026) · cites this paper as [62]

    The Methods section describes a custom-designed, species-specific STR panel, and the Discussion says many panels for animal species identification have been developed. The paper identifies species, not breeds; it does not establish that SNP arrays for breed identification are becoming more common. The Discussion also says that only a few STR-based panels had been used for animal species identification. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

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Cited by

Sorted by citations

Authors
Cui, W., Jin, X., Guo, Y., Chen, C., Zhang, W., Wang, Y., Lan, J., & Zhu, B.
Affiliations
  • Xi'an Jiaotong University (CN)
  • Southern Medical University (CN)
Corresponding author
Zhu, Bofeng
ORCID
Venue
Frontiers in Genetics
Publisher
Frontiers Media SA
Published
2020-09-24
Volume
11
Pages
1005-1005
ISSN
1664-8021 (ISSN-L)
DOI
10.3389/fgene.2020.01005
PMC
PMC7541953
OpenAlex
W3088515807
Cited by
14
References
0
Citations by year
2026: 1 · 2025: 2 · 2024: 2 · 2023: 5 · 2022: 2 · 2021: 2
FWCI
0.73
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Genetics (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
National Natural Science Foundation of China
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Identification and Quantification in Food
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Forensic and Genetic Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
Keywords
multiplex PCR, meat adulteration, animal species identification, personal identification, DNA mixture analysis, species specificity

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