Analytical approaches to examine gamma-aminobutyric acid and glutamate vesicular co-packaging
Open accessAbstract
Multi-transmitter neurons, i.e., those that release more than one type of neurotransmitter, have been found in many organisms and brain areas. Given the peculiar biology of these cells, as well as the potential for diverse effects of each of the transmitters released, new tools, and approaches are necessary to parse the mechanisms and functions of synaptic co-transmission. Recently, we and others have studied neurons that project to the lateral habenula and release both gamma-aminobutyric acid (GABA) and glutamate, in some cases by packaging both transmitters in the same synaptic vesicles. Here, we discuss the main challenges with current electrophysiological approaches to studying the mechanisms of glutamate/GABA co-release, a novel statistical analysis that can identify co-packaging of neurotransmitters versus release from separate vesicle, and the implications of glutamate/GABA co-release for synapse function and plasticity.
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Intersectional genetic and viral tools allow manipulation of specific neuronal populations suspected of neurotransmitter corelease and enable functional analysis of cotransmitting neurons in vitro and in vivo.
“new ''intersectional'' genetic and viral tools permit manipulation of specific neuronal populations suspected of neurotransmitter corelease 10 and enable functional analysis of cotransmitting neurons in vitro and in vivo.11,12”
From Synaptic and circuit functions of multitransmitter neurons in the mammalian brain (2023) · cites this paper as [11]
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References 56 · Cited by 10
- Authors
- Kim, S., & Sabatini, B. L.
- Affiliations
- Howard Hughes Medical Institute (US)
- Corresponding author
- Sabatini, Bernardo L.
- Venue
- Frontiers in Synaptic Neuroscience
- Publisher
- Frontiers Media SA
- Published
- 2023-01-04
- Volume
- 14
- Pages
- 1076616-1076616
- ISSN
- 1663-3563 (ISSN-L)
- PMC
- PMC9846491
- OpenAlex
- W4313585361
- Cited by
- 10
- References
- 56
- Citations by year
- 2025: 1 · 2024: 6 · 2023: 3
- FWCI
- 0.39
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Synaptic Neuroscience (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Institute of Neurological Disorders and Stroke (R37NS046579)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Keywords
- lateral habenula, synaptic vesicles, synaptic function, synaptic plasticity
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