Single-Cell Profiling of Tumor Microenvironment Heterogeneity in Osteosarcoma Identifies a Highly Invasive Subcluster for Predicting Prognosis
Open accessAbstract
Osteosarcoma is the most common malignant bone tumor in adolescents, and metastasis is the key reason for treatment failure and poor prognosis. Once metastasis occurs, the 5-year survival rate is only approximately 20%, and assessing and predicting the risk of osteosarcoma metastasis are still difficult tasks. In this study, cellular communication between tumor cells and nontumor cells was identified through comprehensive analysis of osteosarcoma single-cell RNA sequencing (scRNA-seq) and bulk RNA-seq data, illustrating the complex regulatory network in the osteosarcoma microenvironment. In line with the heterogeneity of osteosarcoma, we found subpopulations of osteosarcoma cells that highly expressed COL6A1, COL6A3 and MIF and were closely associated with lung metastasis. Then, BCDEG, a reliable risk regression model that could accurately assess the metastasis risk and prognosis of patients, was established, providing a new strategy for the diagnosis and treatment of osteosarcoma.
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The seed and soil phenomenon in osteosarcoma is driven by a microenvironment that modulates osteosarcoma cell behaviour and facilitates proliferation, quiescence, invasion, migration and drug resistance, and contributes to intrinsic heterogeneity.
“This seed and soil phenomenon is driven by a microenvironment that modulates osteosarcoma cell behaviour and facilitates proliferation, quiescence, invasion, migration, and drug resistance 15,51,52,53 and contributes to their intrinsic heterogeneity 54,55 .”
From Osteosarcoma (2022) · cites this paper as [55]
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References 59 · Cited by 28
- Authors
- Guo, J., Tang, H., Huang, P., Guo, J., Shi, Y., Yuan, C., Liang, T., & Tang, K.
- Affiliations
- Army Medical University (CN)
- Corresponding author
- Yuan, Chengsong
- Liang, Taotao
- Tang, Kanglai
- Venue
- Frontiers in Oncology
- Publisher
- Frontiers Media SA
- Published
- 2022-04-06
- Volume
- 12
- Pages
- 732862-732862
- ISSN
- 2234-943X (ISSN-L)
- PMC
- PMC9020875
- OpenAlex
- W4225730432
- Cited by
- 28
- References
- 59
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 6 · 2023: 5 · 2022: 10
- FWCI
- 4.03
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Oncology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Army Medical University
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Sarcoma Diagnosis and Treatment
- Health Sciences › Medicine › Rheumatology › Bone Tumor Diagnosis and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
- Keywords
- osteosarcoma, tumor microenvironment heterogeneity, lung metastasis, prognostic model, COL6A1, COL6A3, macrophage migration inhibitory factor, tumor cell subpopulations, single-cell RNA sequencing
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