Enhanced lipid production in Chlamydomonas reinhardtii by co-culturing with Azotobacter chroococcum
Open accessAbstract
The green algae, Chlamydomonas reinhardtii, is one of thea model species used to study for research into lipid production, although research has focused on nitrogen-deficient cultures, which that inhibitions the development of biomass by C. reinhardtii and. It is the key limiting factor in lipid production by C. reinhardtii. In this study, Azotobacter chroococcum was added to the algal culture in order to improve the lipid accumulation and productivity of C. reinhardtii. The maximum lipid content and production of C. reinhardtii in the co-culture were 65.85% and 387.76 mg/L, respectively, which were 2.3-fold and 5.9 times-fold the control’s levels of 29.11% and 65.99 mg/L, respectively. The maximum lipid productivity of C. reinhardtii in the co-culture was 141.86 mg/(L•day), which was 19.4 times-fold the control’s levels of 7.33 mg/(L•day). Theose increases were attributed to the enhanced growth and, biomass and the changeed in the activity of enzymes related to lipid regulation: (ACCase, DGAT, and PDAT). Furthermore, cCompared to the conventional strategy of nitrogen deprivation, A. chroococcum added to the culture of C. reinhardtii resulteds in a higher lipid accumulation and activity, greater efficiency in the conversion of proteins to lipids, higher biomass, and increased growth of C. reinhardtii. In this wayTherefore, using A. chroococcum to improve the growth and biomass of C. reinhardtii is an efficient, rapid, and economically viable strategy for improving enhancing lipid production in the green algae C. reinhardtii.
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Co-cultivation of C. reinhardtii with Azotobacter chroococcum under nitrogen starvation increased lipid content 2.4 times, lipid production 5.9 times, and lipid productivity up to 19.4 times compared with axenic microalga.
“The authors reported an increase of 2.4 times in lipid content and 5.9 times in lipid production with the co-culture and up to 19.4 times the lipid productivity compared with the axenic microalga.”
From Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia (2021) · cites this paper as [83]
C. vulgaris cultivated with Stenotrophomonas maltophilia produced more than 80% C16 and C18 in fatty acids, an increase of up to 5% compared to axenic cultures.
“Xue et al. [83] reported more than 80% content of C16 and C18 in the fatty acids produced by the microalga C. vulgaris when cultivated with Stenotrophomonas maltophilia as well as an increase of up to 5% when compared to axenic cultures.”
From Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia (2021) · cites this paper as [83]
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References 38 · Cited by 77
Research works printed but not linked: 1
- Harris E. H. (2009). The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use. San Diego, CA: Academic Press.
- Authors
- Xu, L., Cheng, X., & Wang, Q.
- Affiliations
- Shanghai Normal University (CN)
- Corresponding author
- Wang, Quanxi
- ORCID
- Venue
- Frontiers in Plant Science
- Publisher
- Frontiers Media SA
- Published
- 2018-06-28
- Volume
- 9
- Pages
- 741-741
- ISSN
- 1664-462X (ISSN-L)
- PMC
- PMC6032324
- OpenAlex
- W2804003354
- Cited by
- 77
- References
- 38
- Citations by year
- 2026: 5 · 2025: 13 · 2024: 10 · 2023: 22 · 2022: 10 · 2021: 7 · 2020: 8 · 2019: 2
- FWCI
- 0.98
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Frontiers in Plant Science (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (31600284)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Chlamydomonas reinhardtii, Azotobacter chroococcum, co-culture, lipid accumulation, nitrogen starvation, nitrogen fixation, algal growth, biomass, acetyl-CoA carboxylase, diacylglycerol acyltransferase, phospholipid:diacylglycerol acyltransferase, microalgae
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