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Cited by 16Phytochemistry and Biological ActivitiesNatural Antidiabetic Agents StudiesPhytochemical Studies and Bioactivities

Comparison of Various Solvent Extracts and Major Bioactive Components from Unsalt-Fried and Salt-Fried Rhizomes of Anemarrhena asphodeloides for Antioxidant, Anti-α-Glucosidase, and Anti-Acetylcholinesterase Activities

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Chu, Yi-Cheng, Yang, Chang-Syun, Cheng, Ming-Jen, Fu, Shu-Ling, Chen, Jih-Jung·2022·Antioxidants
DOI 10.3390/antiox11020385PMC PMC8868586
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Abstract

The rhizome of Anemarrhena asphodeloides Bunge (AA, family Liliaceae) is a famous and frequently used herbal drug in the traditional medicine of Northeast Asia, under vernacular name “zhimu”. A. asphodeloides has been used as an anti-inflammatory, antipyretic, anti-platelet aggregation, anti-depressant, and anti-diabetic agent in traditional Chinese medicine. We examined the antioxidant, anti-acetylcholinesterase (AChE), and anti-α-glucosidase activities of various solvent extracts and the main bioactive compounds from the rhizome of A. asphodeloides. Acetone extract exhibited comparatively high antioxidant activities by 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and ferric-reducing antioxidant power (FRAP) assays. A water extract exhibited relatively strong antioxidant activity by superoxide radical scavenging test. Furthermore, dichloromethane, chloroform, and n-hexane extracts showed significant anti-α-glucosidase activities. Finally, ethanol and dichloromethane extracts exhibited relatively strong AChE inhibitory activity. HPLC analysis was used to examine and compare various solvent extracts for their compositions of isolates. We isolated four major chemical constituents and analyzed their antioxidant, anti-α-glucosidase, and AChE inhibitory activities. The bioactivity assays showed that mangiferin displayed the most potential antioxidant activities via FRAP, ABTS, DPPH, and superoxide assays and also exhibited the most effective anti-AChE and anti-α-glucosidase activities among all the isolates. The present study suggests that A. asphodeloides and its active extracts and components are worth further investigation and might be expected to develop as a candidate for the treatment or prevention of oxidative stress-related diseases, AChE inhibition, and hyperglycemia.

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  • MGF improves memory by enhancing cholinergic transmission through acetylcholinesterase inhibition and NF-κB suppression.

    “It also improves memory by enhancing cholinergic transmission through acetylcholinesterase inhibition and NF-κB suppression (134).”

    From Mangiferin in human disease: Multifaceted mechanisms and applications (Review) (2026) · cites this paper as [134]

    The Discussion reports that mangiferin may improve memory and that this study found anti-acetylcholinesterase activity; the Introduction explains that acetylcholinesterase inhibition can increase cholinergic signaling. The paper does not establish that mangiferin improves memory through that mechanism, and no passage supports NF-κB suppression. (LLM, conf=0.88)

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Cited by

Sorted by citations

Authors
Chu, Y.-C., Yang, C.-S., Cheng, M.-J., Fu, S.-L., & Chen, J.-J.
Affiliations
  • National Yang Ming Chiao Tung University (TW)
  • Food Industry Research and Development Institute (TW)
Corresponding author
  • Fu, Shu-Ling
  • Chen, Jih-Jung
ORCID
Venue
Antioxidants
Publisher
MDPI AG
Published
2022-02-14
Volume
11
Issue
2
Pages
385
ISSN
2076-3921 (ISSN-L)
DOI
10.3390/antiox11020385
PMC
PMC8868586
OpenAlex
W4212999657
Cited by
16
References
1
Citations by year
2025: 3 · 2024: 3 · 2023: 5 · 2022: 5
FWCI
2.20
Type
journal article
Language
en
Open access
Licence
Locations
  • Antioxidants (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Natural Antidiabetic Agents Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical Studies and Bioactivities
Keywords
Anemarrhena asphodeloides, rhizome, solvent extracts, mangiferin, antioxidant activity, anti-α-glucosidase activity, anti-acetylcholinesterase activity, traditional Chinese medicine, oxidative stress, hyperglycemia, bioactive compounds

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