Gene Delivery Technologies with Applications in Microalgal Genetic Engineering
Open accessAbstract
Microalgae and cyanobacteria are photosynthetic microbes that can be grown with the simple inputs of water, carbon dioxide, (sun)light, and trace elements. Their engineering holds the promise of tailored bio-molecule production using sustainable, environmentally friendly waste carbon inputs. Although algal engineering examples are beginning to show maturity, severe limitations remain in the transformation of multigene expression cassettes into model species and DNA delivery into non-model hosts. This review highlights common and emerging DNA delivery methods used for other organisms that may find future applications in algal engineering.
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Despite these obstacles, electroporation remains an important technique for introducing plasmid DNA into specific cyanobacterial strains, where alternative methods may be less effective.
“Despite these obstacles, electroporation remains an important technique for introducing plasmid DNA into specific cyanobacterial strains, where alternative methods may be less effective [85,86].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [86]
Section 2.2 (Electroporation; page unavailable) reports that electroporation delivers DNA and has transformed cyanobacteria including Anabaena and Nostoc punctiforme. Section 3.1 (Natural Transformation; page unavailable) says natural transformation is limited to species with that native ability. The copy does not specifically establish electroporation of plasmid DNA in those strains or show that alternative methods are less effective for them. (LLM, conf=0.83)
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References 179 · Cited by 68
- Authors
- Gutiérrez, S., & Lauersen, K. J.
- Affiliations
- King Abdullah University of Science and Technology (SA)
- Corresponding author
- Lauersen, Kyle J.
- Venue
- Biology
- Publisher
- MDPI AG
- Published
- 2021-03-26
- Volume
- 10
- Issue
- 4
- Pages
- 265
- ISSN
- 2079-7737 (ISSN-L)
- PMC
- PMC8067306
- OpenAlex
- W3149267289
- Cited by
- 68
- References
- 179
- Citations by year
- 2026: 11 · 2025: 13 · 2024: 21 · 2023: 10 · 2022: 9 · 2021: 4
- FWCI
- 0.54
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Biology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- King Abdullah University of Science and Technology Repository (King Abdullah University of Science and Technology) (submittedVersion, other-oa)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Keywords
- gene delivery, cyanobacteria, DNA transformation, photosynthetic microorganisms
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