The DOI matches its Crossref record; 67 of the 67 references it cites were found in a registry or an index.
Refolding Techniques for Recovering Biologically Active Recombinant Proteins from Inclusion Bodies
Abstract
Biologically active proteins are useful for studying the biological functions of genes and for the development of therapeutic drugs and biomaterials in a biotechnology industry. Overexpression of recombinant proteins in bacteria, such as Escherichia coli, often results in the formation of inclusion bodies, which are protein aggregates with non-native conformations. As inclusion bodies contain relatively pure and intact proteins, protein refolding is an important process to obtain active recombinant proteins from inclusion bodies. However, conventional refolding methods, such as dialysis and dilution, are time consuming and, often, recovered yields of active proteins are low, and a trial-and-error process is required to achieve success. Recently, several approaches have been reported to refold these aggregated proteins into an active form. The strategies largely aim at reducing protein aggregation during the refolding procedure. This review focuses on protein refolding techniques using chemical additives and laminar flow in microfluidic chips for the efficient recovery of active proteins from inclusion bodies.
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Cites
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Cited by
Sarker, A., Rathore, A. S., & Gupta, R. D. (2019) · Microbial Cell Factories · doi:10.1186/s12934-019-1053-9 · Cited by 75
The master energy homeostasis regulator PGC-1α exhibits an mRNA nuclear export function
Mihaylov, S. R., Castelli, L. M., Lin, Y.-H., Gül, A., Soni, N., Hastings, C., Flynn, H. R., Păun, O., Dickman, M. J., Snijders, A. P., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-41304-8 · Cited by 63
Advances in monitoring and control of refolding kinetics combining PAT and modeling
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Metadata
- Authors
- Yamaguchi, H., & Miyazaki, M.
- Venue
- Biomolecules
- Publisher
- MDPI AG
- Published
- 2014-02-20
- Volume
- 4
- Issue
- 1
- Pages
- 235-251
- ISSN
- 2218-273X
- Cited by
- 251
- References
- 67
- Language
- en
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- Yamaguchi, H., & Miyazaki, M. (2014). Refolding Techniques for Recovering Biologically Active Recombinant Proteins from Inclusion Bodies. Biomolecules. https://doi.org/10.3390/biom4010235
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