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Enhanced Electrocatalytic Properties of Co3O4 Nanocrystals Derived from Hydrolyzed Polyethyleneimines in Water/Ethanol Solvents for Electrochemical Detection of Cholesterol

Alshgari, Razan A., Nafady, Ayman, Shah, Aqeel Ahmed, Aboelmaaref, Amal, Aftab, Umair, Ibupoto, Mazhar Hussain, Vigolo, Brigitte, Tahira, Aneela, Ibupoto, Zafar Hussain·2022·Catalysts
DOI 10.3390/catal12101176
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Abstract

The present study describes the effect of hydrolysis of polyethyleneimines in water/ethanol mixture on the morphology of the cobalt oxide (Co3O4), used as the main sensor component. The structure of the generated Co3O4 nanocrystals is consistent with a well-defined cubic phase crystallography, having only cobalt and oxygen elements. Developing simple, low-cost, sensitive, and selective cholesterol biosensors is essential for accurate monitoring of cholesterol to avoid cardiovascular diseases. These nanocrystals exhibit large surfaces suitable for facile and high loading of cholesterol oxidase enzyme through the physical adsorption method. Then, the fabricated cholesterol oxidase/ Co3O4 nanocrystals composite was implemented for potentiometric detection of cholesterol in 10 mM phosphate buffer of pH 7.3. Importantly, the presented cholesterol biosensor revealed a wide linear range of 0.005 mM to 3.0 mM with a limit of detection (LOD) of 0.001 mM. Additionally, the sensitivity of biosensor was estimated around 60 mVdec−1. The selectivity, stability, reproducibility, and repeatability were also observed as satisfactory. The dynamic response of the proposed method demonstrated a fast response time of less than 1 s. Furthermore, the successive addition method confirmed a remarkably stable response towards various cholesterol concentrations. Thus, the developed cholesterol oxidase/ Co3O4 nanocomposite may be used as an efficient alternative method to monitor low cholesterol concentrations form real samples.

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Metadata

Authors
Alshgari, R. A., Nafady, A., Shah, A. A., Aboelmaaref, A., Aftab, U., Ibupoto, M. H., Vigolo, B., Tahira, A., & Ibupoto, Z. H.
Venue
Catalysts
Publisher
MDPI AG
Published
2022-10-05
Volume
12
Issue
10
Pages
1176
ISSN
2073-4344
DOI
10.3390/catal12101176
Cited by
2
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (vor)
Funders
King Saud University (Riyadh, Saudi Arabia) through the Researchers Supporting Project (RSP-2022/265)

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CoChat. (2026). Citation check: Enhanced Electrocatalytic Properties of Co3O4 Nanocrystals Derived from Hydrolyzed Polyethyleneimines in Water/Ethanol Solvents for Electrochemical Detection of Cholesterol. https://app.cochat.ai/papers/10.3390/catal12101176
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Alshgari, R. A., Nafady, A., Shah, A. A., Aboelmaaref, A., Aftab, U., Ibupoto, M. H., Vigolo, B., Tahira, A., & Ibupoto, Z. H. (2022). Enhanced Electrocatalytic Properties of Co3O4 Nanocrystals Derived from Hydrolyzed Polyethyleneimines in Water/Ethanol Solvents for Electrochemical Detection of Cholesterol. Catalysts. https://doi.org/10.3390/catal12101176

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