The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR?
Open accessAbstract
In 1998, when the PCR technique was already popular, a Japanese company called Eiken Chemical Co., Ltd. designed a method known as the loop-mediated isothermal amplification of DNA (LAMP). The method can produce up to 109 copies of the amplified DNA within less than an hour. It is also highly specific due to the use of two to three pairs of primers (internal, external, and loop), which recognise up to eight specific locations on the DNA or RNA targets. Furthermore, the Bst DNA polymerase most used in LAMP shows a high strand displacement activity, which eliminates the DNA denaturation stage. One of the most significant advantages of LAMP is that it can be conducted at a stable temperature, for instance, in a dry block heater or an incubator. The products of LAMP can be detected much faster than in standard techniques, sometimes only requiring analysis with the naked eye. The following overview highlights the usefulness of LAMP and its effectiveness in various fields; it also considers the superiority of LAMP over PCR and presents RT-LAMP as a rapid diagnostic tool for SARS-CoV-2.
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Cites
Loop-mediated isothermal amplification of DNA
Notomi, T. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.12.e63 · Cited by 8,805
Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
Corman, V. M., Landt, O., Kaiser, M., Molenkamp, R., Meijer, A., Chu, D. K., Bleicker, T., Brünink, S., Schneider, J., Schmidt, M. L., et al. (2020) · Eurosurveillance · doi:10.2807/1560-7917.es.2020.25.3.2000045 · Cited by 7,344
CRISPR–Cas12-based detection of SARS-CoV-2
Broughton, J. P., Deng, X., Yu, G., Fasching, C. L., Servellita, V., Singh, J., Miao, X., Streithorst, J. A., Granados, A., Sotomayor-Gonzalez, A., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0513-4 · Cited by 2,717
Accelerated reaction by loop-mediated isothermal amplification using loop primers
Nagamine, K., Hase, T., & Notomi, T. (2002) · Molecular and Cellular Probes · doi:10.1006/mcpr.2002.0415 · Cited by 2,045
Mori, Y., Nagamine, K., Tomita, N., & Notomi, T. (2001) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2001.5921 · Cited by 1,711
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Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances
Kaneko, H., Kawana, T., Fukushima, E., & Suzutani, T. (2007) · Journal of Biochemical and Biophysical Methods · doi:10.1016/j.jbbm.2006.08.008 · Cited by 707
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A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples
Dao Thi, V. L., Herbst, K., Boerner, K., Meurer, M., Kremer, L. P., Kirrmaier, D., Freistaedter, A., Papagiannidis, D., Galmozzi, C., Stanifer, M. L., et al. (2020) · Science Translational Medicine · doi:10.1126/scitranslmed.abc7075 · Cited by 673
Cited by
CRISPR/Cas9 therapeutics: progress and prospects
Li, T., Yang, Y., Qi, H., Cui, W., Zhang, L., Fu, X., He, X., Liu, M., Li, P.-F., & Yu, T. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01309-7 · Cited by 719
A review of modern methods for the detection of foodborne pathogens
Aladhadh, M. (2023) · Microorganisms · doi:10.3390/microorganisms11051111 · Cited by 233
Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections
Olie, S. E., Andersen, C. Ø., van de Beek, D., & Brouwer, M. C. (2024) · Clinical Microbiology Reviews · doi:10.1128/cmr.00021-24 · Cited by 38
“A disadvantage of LAMP is that, while the execution of the reaction is relatively easy, primer design is complex, which can complicate the devel opment of novel assays (22, 23).”
Metadata
- Authors
- Soroka, M., Wasowicz, B., & Rymaszewska, A.
- Venue
- Cells
- Publisher
- MDPI AG
- Published
- 2021-07-29
- Volume
- 10
- Issue
- 8
- Pages
- 1931
- ISSN
- 2073-4409 (ISSN-L)
- OpenAlex
- W3184781691
- Cited by
- 473
- References
- 52
- FWCI
- 17.22
- Type
- journal article
- Language
- en
- Open access
- gold
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