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Cited by 8Plant tissue culture and regenerationGenetic diversity and population structureGrowth and nutrition in plants

Clonal Fidelity and Genetic Diversity of Micropropagated Hancornia speciosa Gomes (Apocynaceae) as Evaluated by Molecular Markers

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Costa, Géssica Ferreira da, Cabral, Pablo Diego Silva, Silva, Fabiano Guimarães, Rubio Neto, Aurélio, Mendonça, Maria Andréia Corrêa·2022·Forests
DOI 10.3390/f13101645
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Abstract

The plant tissue culture procedures for asexual multiplication of Hancornia speciosa represent an important process facilitating the preservation of selected genotypes of this threatened species and large-scale production of plantlets for population reinforcement or other utilization. However, there is no research regarding the somaclonal variation of this threatened species at molecular level. This study aimed to evaluate the role of the multiplication process in the genetic stability of H. speciosa plantlets and calluses using molecular markers. The tissue culture techniques for H. speciosa micropropagation used in this study did not influence the occurrence of somaclonal variation, which can be related to the genotypes of the donor plants and to the low concentrations of growth regulators used in tissue culture procedures adopted. The results observed here provide a reliable methodology for further studies involving micropropagation techniques with the goal of preserving selected or endangered genotypes of H. speciosa without genetic losses and producing seedlings of this species on a large scale. In addition, it was possible to verify that the donor plants used in this study showed a relatively low genetic variability.

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  • Micropropagation is an effective biotechnology technique used in horticulture, forestry, and agriculture that allows rapid and large-scale reproduction of true-to-type, disease-free, and genetically superior varieties.

    “Micropropagation is an effective biotechnology technique used in the horticulture, forestry, and agriculture sectors that allows for the rapid and largescale reproduction of traits that are true-to-type, disease-free, and genetically superior varieties [12,13].”

    From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [13]

    The Introduction (p. 2) says micropropagation can rapidly multiply plants with desirable characteristics on a large scale under adequate phytosanitary conditions. The Conclusions report genetic fidelity for H. speciosa under the study's conditions. The paper does not establish that the resulting plants are disease-free or genetically superior, or document use across all three named sectors. (LLM, conf=0.85)

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Authors
Costa, G. F. D., Cabral, P. D. S., Silva, F. G., Rubio Neto, A., & Mendonça, M. A. C.
Affiliations
  • Instituto Federal Goiano (BR)
  • Goiano Federal Institute, Rio Verde Campus, Rio Verde 75900-000, GO, Brazil
Corresponding author
Mendonça, Maria Andréia Corrêa
ORCID
Venue
Forests
Publisher
MDPI AG
Published
2022-10-08
Volume
13
Issue
10
Pages
1645
ISSN
1999-4907 (ISSN-L)
DOI
10.3390/f13101645
OpenAlex
W4303685852
Cited by
8
References
76
Citations by year
2026: 1 · 2025: 3 · 2024: 3 · 2023: 1
FWCI
0.57
Type
journal article
Language
en
Open access
Licence
Locations
  • Forests (publishedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
  • Fundação de Amparo à Pesquisa do Estado de Goiás
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Growth and nutrition in plants
Keywords
Hancornia speciosa, Apocynaceae, micropropagation, somaclonal variation, clonal fidelity, genetic diversity, genetic stability, threatened plant species, in vitro conservation, plantlets, callus

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