In vitro bile salt hydrolase (BSH) activity screening of different probiotic microorganisms
Open accessAbstract
Bile salt hydrolase (BSH) activity in probiotic strains is usually correlated with the ability to lower serum cholesterol levels in hypercholesterolemic patients. The objective of this study was the evaluation of BSH in five probiotic strains of lactic acid bacteria (LAB) and a probiotic yeast. The activity was assessed using a qualitative direct plate test and a quantitative high-performance thin- layer chromatography assay. The six strains differed in their BSH substrate preference and activity. Lactobacillus plantarum DGIA1, a potentially probiotic strain isolated from a double cream cheese from Chiapas, Mexico, showed excellent deconjugation activities in the four tested bile acids (69, 100, 81, and 92% for sodium glycocholate, glycodeoxycholate, taurocholate, and taurodeoxycholate, respectively). In the case of the commercial probiotic yeast Saccharomyces boulardii, the deconjugation activities were good against sodium glycodeoxycholate, taurocholate, and taurodeoxycholate (100, 57, and 63%, respectively). These last two results are part of the novelty of the work. A weak deconjugative activity (5%) was observed in the case of sodium glycocholate. This is the first time that the BSH activity has been detected in this yeast.
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Secretion of bile salt hydrolase by Lactobacillus spp. contributes to the reduction in host cholesterol levels by hydrolyzing bound bile acids, thereby preventing their absorption.
“the secretion of bile salt hydrolase (BSH) by Lactobacillus spp. contributes to the reduction in host cholesterol levels by hydrolyzing bound bile acids within the organism, thereby preventing their absorption (Hernández-Gómez et al., 2021).”
From Lactiplantibacillus plantarum N4 ameliorates lipid metabolism and gut microbiota structure in high fat diet-fed rats (2024) · cites this paper as Hernández-Gómez et al., 2021
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References 45 · Cited by 122
- Authors
- Hernández-Gómez, J. G., López-Bonilla, A., Trejo-Tapia, G., Ávila-Reyes, S. V., Jiménez-Aparicio, A. R., & Hernández-Sánchez, H.
- Affiliations
- Instituto Politécnico Nacional (MX)
- Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Yautepec CP 62731, Mexico
- Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu 399, UP Adolfo López Mateos, Mexico City CP 07738, Mexico
- ORCID
- Venue
- Foods
- Publisher
- MDPI AG
- Published
- 2021-03-22
- Volume
- 10
- Issue
- 3
- Pages
- 674
- ISSN
- 2304-8158 (ISSN-L)
- PMC
- PMC8004636
- OpenAlex
- W3139493838
- Cited by
- 122
- References
- 45
- Citations by year
- 2026: 21 · 2025: 26 · 2024: 24 · 2023: 20 · 2022: 25 · 2021: 6
- FWCI
- 11.21
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Foods (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Instituto Politécnico Nacional (SIP-20200277)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- bile salt hydrolase, probiotics, lactic acid bacteria, Saccharomyces boulardii, Lactobacillus plantarum, bile acid deconjugation, cholesterol lowering, serum cholesterol, sodium glycodeoxycholate, taurocholate, taurodeoxycholic acid, sodium glycocholate
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