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Salt Stress Accelerates Tyrosine Depletion by Enterococcus lactis KUST2812: Multi-Omics Insights into Metabolic Remodeling

Gong, Xiaoqi, Ma, Qingyu, Zhong, Yujie, Liu, Zhijia, Chu, Chuanqi, Yi, Junjie, Wang, Tao·2026·Foods
DOI 10.3390/foods15183190
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Abstract

Enterococcus lactis KUST2812 is a halotolerant strain isolated from broad bean paste (BBP). It shows strong potential as a safe fermentation starter due to its high survival rate (98.32% in 18% NaCl), absence of hemolytic activity, and susceptibility to clinically relevant antibiotics, with resistance limited to intrinsic traits. Growth and metabolic dynamics suggest that salt stress decouples growth from tyrosine catabolism: while 6% NaCl significantly inhibited bacterial cell growth, tyrosine depletion occurred within 12 h under 6% NaCl, compared with the 96 h required under non-saline conditions. Multi-omics analyses suggest a molecular network associated with this tyrosine conversion under salt stress. Two tyrosine decarboxylase genes (tdc) were upregulated by 1.85- and 6.50-fold, respectively. The tyrosine-specific transporter gene tyrP was upregulated 4.99-fold, and the protein synthesis-associated gene tyrS was upregulated 4.93-fold. These changes collectively support a diversified tyrosine utilization strategy of E. lactis KUST2812. Moreover, coordinated responses involving the Na+/H+ antiporter system, compatible solute transporters, and oxidative stress markers contributed to the metabolic basis for salt adaptation of this strain. Evaluation in BBP fermentation suggested that E. lactis KUST2812 may reduce tyrosine accumulation without causing a significant increase in tyramine. These findings offer a potential microbial resource and a theoretical basis for managing tyrosine-related quality issues in fermented foods.

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Research works printed but not linked: 53

  1. Chen, 2025, Effect of stir-frying temperature on volatile aroma compounds in Pixian broad bean paste, Int. J. Gastron. Food Sci., 40, 101156, 10.1016/j.ijgfs.2025.101156 · DOI
  2. Zhao, 2024, Insight into the keystones of Chinese broad bean paste fermentation: Brewing techniques, chemosensory characteristics, and microbial community, Trends Food Sci. Technol., 154, 104775, 10.1016/j.tifs.2024.104775 · DOI
  3. Niu, 2026, Changing trends of non-volatile metabolites during industrial broad bean paste production via untargeted metabolomics, Food Res. Int., 223, 117926, 10.1016/j.foodres.2025.117926 · DOI
  4. Xie, 2022, Impact of salt concentration on bacterial diversity and changes in biogenic amines during fermentation of farmhouse soybean paste in Northeast China, Curr. Res. Food Sci., 5, 1225, 10.1016/j.crfs.2022.07.012 · DOI
  5. Duan, 2016, Composition and metabolic activities of the bacterial community in shrimp sauce at the flavor-forming stage of fermentation as revealed by metatranscriptome and 16S rRNA gene sequencings, J. Agric. Food Chem., 64, 2591, 10.1021/acs.jafc.5b05826 · DOI
  6. Xiang, 2024, Revealing the formation mechanisms of key flavors in fermented broad bean paste, Food Res. Int., 177, 113880, 10.1016/j.foodres.2023.113880 · DOI
  7. Niu, 2018, Adaptive evolution of Aspergillus oryzae 3.042 strain and process optimization to reduce the formation of tyrosine crystals in broad bean paste, J. Food Biochem., 43, e12716
  8. Min, 2020, Composition, source, and influencing factors of white spots formation in soybean paste, J. Food Sci., 85, 3113, 10.1111/1750-3841.15457 · DOI
  9. Deng, 2024, A model to reduce tyrosine crystallization in Chinese fermented soybean curd by adjusting NaCl, ethanol and temperature during fermentation, Food Qual. Saf., 8, fyad055, 10.1093/fqsafe/fyad055 · DOI
  10. Limbo, 2016, New insight on crystal and spot development in hard and extra-hard cheeses: Association of spots with incomplete aggregation of curd granules, J. Dairy Sci., 99, 6144, 10.3168/jds.2016-11050 · DOI · Paper page
  11. Gou, 2021, Processing parameters involved in the development of texture and tyrosine precipitates in dry-cured ham: Modelisation of texture development, Meat Sci., 172, 108362, 10.1016/j.meatsci.2020.108362 · DOI
  12. Rudolph, 2018, Studies on the interaction of the aromatic amino acids tryptophan, tyrosine and phenylalanine as well as tryptophan-containing dipeptides with cyclodextrins, Eur. Food Res. Technol., 244, 1511, 10.1007/s00217-018-3065-9 · DOI
  13. Gong, 2026, Effects of a novel salt-tolerance Lactiplantibacillus plantarum on the physicochemical properties and volatile profiles of broad-bean paste, Food Chem. X, 34, 103598, 10.1016/j.fochx.2026.103598 · DOI
  14. Xu, 2020, Construction of a heat-inducible Escherichia coli strain for efficient de novo biosynthesis of L-tyrosine, Process Biochem., 92, 85, 10.1016/j.procbio.2020.02.023 · DOI
  15. Zhou, 2024, A synergistic fermentation system of probiotics with low-cost and high butyric acid production: Lactiplantibacillus plantarum and Clostridium tyrobutyricum, Food Biosci., 62, 105152, 10.1016/j.fbio.2024.105152 · DOI
  16. Li, 2023, Screening lactic acid bacteria and yeast strains for soybean paste fermentation in northeast of China, Food Sci. Nutr., 11, 4502, 10.1002/fsn3.3372 · DOI
  17. Shi, 2020, Evaluation of safety and probiotic properties of a strain of Enterococcus faecium isolated from chicken bile, J. Food Sci. Technol. Mysore, 57, 578, 10.1007/s13197-019-04089-7 · DOI
  18. Lin, 2025, Unravelling the response mechanism of extracellular polysaccharides synthesis and salt stress resistance in Tetragenococcus halophilus JY1biofilm formation, Food Biosci., 66, 106327, 10.1016/j.fbio.2025.106327 · DOI
  19. Zhou, 2025, Integrated transcriptomic and metabolomic analyses reveal the mechanism of Lactiplantibacillus plantarum enhancing butyric acid production by Clostridium tyrobutyricum, Food Biosci., 71, 107418, 10.1016/j.fbio.2025.107418 · DOI
  20. Zhao, 2026, Tricholoma matsutake polysaccharides promote physiological germination and metabolic reactivation of Bacillus cereus spores: Mechanisms and application in milk preservation, Int. J. Food Microbiol., 460, 111936, 10.1016/j.ijfoodmicro.2026.111936 · DOI
  21. Li, 2021, The microbial community, biogenic amines content of soybean paste, and the degradation of biogenic amines by Lactobacillus plantarum HM24, Food Sci. Nutr., 9, 6458, 10.1002/fsn3.2528 · DOI
  22. Pereira, 2009, Dual role for the tyrosine decarboxylation pathway in Enterococcus faecium E17: Response to an acid challenge and generation of a proton motive force, Appl. Environ. Microbiol., 75, 345, 10.1128/AEM.01958-08 · DOI · Paper page
  23. Gatto, 2016, Tyrosine decarboxylase activity of Enterococcus mundtii: New insights into phenotypic and genetic aspects, Microb. Biotechnol., 9, 801, 10.1111/1751-7915.12402 · DOI
  24. Saelao, 2018, Reduction of tyramine accumulation in Thai fermented shrimp (kung-som) by nisin Z-producing Lactococcus lactis KTH0-1S as starter culture, Food Control, 90, 249, 10.1016/j.foodcont.2018.03.003 · DOI
  25. Goszcz, 2025, Bacterial osmoprotectants-a way to survive in saline conditions and potential crop allies, FEMS Microbiol. Rev., 49, fuaf020, 10.1093/femsre/fuaf020 · DOI
  26. Yao, 2020, Identification of salt tolerance-related genes of Lactobacillus plantarum D31 and T9 strains by genomic analysis, Ann. Microbiol., 70, 10, 10.1186/s13213-020-01551-2 · DOI
  27. Mahan, 1983, Genetic analysis of the proBA genes of Salmonella typhimurium: Physical and genetic analyses of the cloned proB+ A+ genes of Escherichia coli and of a mutant allele that confers proline overproduction and enhanced osmotolerance, J. Bacteriol., 156, 1249, 10.1128/jb.156.3.1249-1262.1983 · DOI
  28. Thakur, 2024, Biofilm forming, exopolysaccharide producing and halotolerant, bacterial consortium mitigates salinity stress in Triticum aestivum, Int. J. Biol. Macromol., 262, 130049, 10.1016/j.ijbiomac.2024.130049 · DOI
  29. Liu, 2024, β-Hemolysin, not agrA mutation, inhibits the hemolysis of α-hemolysin in Staphylococcus aureus laboratory and clinical strains, mSphere, 9, e00673-23, 10.1128/msphere.00673-23 · DOI
  30. Li, 2025, Synthesis and antibacterial evaluations of novel vancomycin analogues targeting bacteria membrane to combat Gram-negative infections, Eur. J. Med. Chem., 289, 117483, 10.1016/j.ejmech.2025.117483 · DOI
  31. Aqib, A.I., and Alsayeqh, A.F. (2022). Vancomycin drug resistance, an emerging threat to animal and public health. Front. Vet. Sci., 9. · DOI
  32. Adeyemi, F.M., Oyedara, O.O., Yusuf-Omoloye, N.A., Ajigbewu, O.H., Ndaji, O.L., Adegbite-Badmus, M.K., Olumakinde, T.S., and Oluokun, T.E. (2024). Guardians of resistance and virulence: Detection of mec, femA, Van, pvl, hlg and spa genes in methicillin and vancomycin-resistant Staphylococcus aureus from clinical and food samples in Southwestern Nigeria. BMC Microbiol., 24. · DOI
  33. Haryani, 2022, High prevalence of multiple antibiotic resistance in fermented food-associated lactic acid bacteria in Malaysia, Food Control, 147, 109558, 10.1016/j.foodcont.2022.109558 · DOI
  34. Tadesse, 2025, Genome analysis reveals a biased distribution of virulence and antibiotic resistance genes in the genus Enterococcus and an abundance of safe species, Appl. Environ. Microbiol., 91, e00415-25, 10.1128/aem.00415-25 · DOI
  35. Zhu, 2025, Safety assessment of Enterococcus lactis B3 isolated from the traditional Chinese fermented food Bianba and its effect on the quality change in radish paocai, LWT, 226, 117999, 10.1016/j.lwt.2025.117999 · DOI
  36. Roer, 2024, VirulenceFinder for Enterococcus faecium and Enterococcus lactis: An enhanced database for detection of putative virulence markers by using whole-genome sequencing data, Microbiol. Spectr., 12, e03724-23, 10.1128/spectrum.03724-23 · DOI
  37. Yoon, J.-H., Kim, D., Joung, H., and Lee, S.-Y. (2025). The habituation to different concentrations of salt may variably influence the ability of Cronobacter sakazakii, Salmonella enterica serovar Enteritidis, Bacillus cereus, and Staphylococcus aureus to resist acid, bile salt, and heat stresses. Food Microbiol., 128. · DOI
  38. Liu, Y., Hu, Y., Huang, D., Bayinbate, B., Zheng, S., and Xu, B. (2025). The effects of fermented sausage quality driven by reduction of NaCl: Investigation into the microbial community and flavor profiles. Food Res. Int., 204. · DOI
  39. Yu, 2022, Physiological metabolic topology analysis of Halomonas elongata DSM 2581T in response to sodium chloride stress, Biotechnol. Bioeng., 119, 3509, 10.1002/bit.28222 · DOI
  40. Loganathan, 2025, Biological mitigation of salt stress: Role of halophilic bacteria in exopolysaccharides (EPS) and indole-3-acetic acid (IAA) biosynthesis, Int. Microbiol., 29, 61, 10.1007/s10123-025-00768-y · DOI
  41. Zhang, L., Zhu, C., Chen, X., Xu, X., and Wang, H. (2020). Resistance of detached-cells of biofilm formed by Staphylococcus aureus to ultra high pressure homogenization. Food Res. Int., 139. · DOI
  42. Zheng, S., Li, Y., Shao, Y., Li, L., and Song, F. (2024). Osmotic pressure and its biological implications. Int. J. Mol. Sci., 25. · DOI
  43. Wang, A., Du, Q., Li, X., Cui, Y., Luo, J., Li, C., Peng, C., Zhong, X., and Huang, G. (2024). Intracellular and extracellular metabolic response of the Lactic acid bacterium Weissella confusa under salt stress. Metabolites, 14. · DOI
  44. Wang, X.J., Lu, J.Z., Yu, D., Tao, S.Y., Zhang, Y., Li, J.Y., and Yu, J.X. (2026). Fermented orange peel mitigates biogenic amines in Huangjiu: Insights into flavonoid-histidine/tyrosine decarboxylase interactions. LWT, 243. · DOI
  45. Wolken, 2006, The mechanism of the tyrosine transporter TyrP supports a proton motive tyrosine decarboxylation pathway in Lactobacillus brevis, J. Bacteriol., 188, 2198, 10.1128/JB.188.6.2198-2206.2006 · DOI · Paper page
  46. Kageyama, 2024, Novel NhaC Na+/H+ antiporter in cyanobacteria contributes to key molecular processes for salt tolerance, Plant Mol. Biol., 114, 111, 10.1007/s11103-024-01510-4 · DOI
  47. Nie, T.Y., Wang, L.Q., Liu, Y.L., Fu, S.Q., Wang, J.H., Cui, K.P., and Wang, L. (2025). A halophilic bacterium for bioremediation of saline-alkali land: The triadic and rynergetic response mechanism of Oceanobacillus picturae DY09 to Salt Stress. Microorganisms, 13. · DOI
  48. Galinier, A., Delan-Forino, C., Foulquier, E., Lakhal, H., and Pompeo, F. (2023). Recent advances in peptidoglycan synthesis and regulation in bacteria. Biomolecules, 13. · DOI
  49. Li, 2017, Cryptococcus neoformans UGT1 encodes a UDP-Galactose/UDP-GalNAc transporter, Glycobiology, 27, 87, 10.1093/glycob/cww078 · DOI
  50. Liu, 2012, Characterization of RNA damage under oxidative stress in Escherichia coli, Biol. Chem., 393, 123, 10.1515/hsz-2011-0247 · DOI
  51. Cobb, 2016, α-Hydroxybutyric acid is a selective metabolite biomarker of impaired glucose tolerance, Diabet. Care, 39, 988, 10.2337/dc15-2752 · DOI
  52. Chen, 2020, Glycerol, trehalose and vacuoles had relations to pullulan synthesis and osmotic tolerance by the whole genome duplicated strain Aureobasidium melanogenum TN3-1 isolated from natural honey, Int. J. Biol. Macromol., 165, 131, 10.1016/j.ijbiomac.2020.09.149 · DOI
  53. Natrella, G., Vacca, M., Minervini, F., Faccia, M., and De Angelis, M. (2024). A comprehensive review on the biogenic amines in cheeses: Their origin, chemical characteristics, hazard and reduction strategies. Foods, 13. · DOI

Metadata

Authors
Gong, X., Ma, Q., Zhong, Y., Liu, Z., Chu, C., Yi, J., & Wang, T.
Venue
Foods
Publisher
MDPI AG
Published
2026-09-09
Volume
15
Issue
18
Pages
3190
ISSN
2304-8158
DOI
10.3390/foods15183190
Cited by
0
References
1
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (vor)
Funders
  • National Natural Science Foundation of China (32660622)
  • Yunnan Provincial “Revitalize Yunnan Talent Support Program”—Innovation Team for Fruit and Vegetable Processing, Nutrition & Health (202605AS350002)
  • Special Basic Cooperative Research Programs of Yunnan Provincial Undergraduate Universities’ Association (202401BA070001-036)

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CoChat. (2026). Citation check: Salt Stress Accelerates Tyrosine Depletion by Enterococcus lactis KUST2812: Multi-Omics Insights into Metabolic Remodeling. https://app.cochat.ai/papers/10.3390/foods15183190
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Gong, X., Ma, Q., Zhong, Y., Liu, Z., Chu, C., Yi, J., & Wang, T. (2026). Salt Stress Accelerates Tyrosine Depletion by Enterococcus lactis KUST2812: Multi-Omics Insights into Metabolic Remodeling. Foods. https://doi.org/10.3390/foods15183190

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