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Cited by 9Osteoarthritis Treatment and MechanismsHydrogels: synthesis, properties, applicationsTissue Engineering and Regenerative Medicine

PEG Reinforced Scaffold Promotes Uniform Distribution of Human MSC-Created Cartilage Matrix

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Riewruja, Kanyakorn, Aguglia, Alyssa M., Hines, Sophie, Makarcyzk, Meagan J., Honsawek, Sittisak, Lin, Hang·2022·Gels
DOI 10.3390/gels8120794PMC PMC9778361
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Abstract

Previously, we used a gelatin/hyaluronic acid (GH)-based scaffold to induce chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells (hBMSC). The results showed that hBMSCs underwent robust chondrogenesis and facilitated in vivo cartilage regeneration. However, it was noticed that the GH scaffolds display a compressive modulus that is markedly lower than native cartilage. In this study, we aimed to enhance the mechanical strength of GH scaffolds without significantly impairing their chondrosupportive property. Specifically, polyethylene glycol diacrylate (PEGDA) and photoinitiators were infiltrated into pre-formed hBMSC-laden GH scaffolds and then photo-crosslinked. Results showed that infiltration of PEG at the beginning of chondrogenesis significantly increased the deposition of glycosaminoglycans (GAGs) in the central area of the scaffold. To explore the mechanism, we compared the cell migration and proliferation in the margin and central areas of GH and PEG-infiltrated GH scaffolds (GH+PEG). Limited cell migration was noticed in both groups, but more proliferating cells were observed in GH than in GH+PEG. Lastly, the in vitro repairing study with bovine cartilage explants showed that PEG- impregnated scaffolds integrated well with host tissues. These results indicate that PEG-GH hybrid scaffolds, created through infiltrating PEG into pre-formed GH scaffolds, display good integration capacity and represent a new tool for the repair of chondral injury.

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Cited by

Sorted by citations

Authors
Riewruja, K., Aguglia, A. M., Hines, S., Makarcyzk, M. J., Honsawek, S., & Lin, H.
Affiliations
  • Thai Red Cross Society (TH)
  • University of Pittsburgh (US)
Corresponding author
Lin, Hang
ORCID
Venue
Gels
Publisher
MDPI AG
Published
2022-12-03
Volume
8
Issue
12
Pages
794
ISSN
2310-2861 (ISSN-L)
DOI
10.3390/gels8120794
PMC
PMC9778361
OpenAlex
W4311628254
Cited by
9
References
0
Citations by year
2026: 2 · 2025: 3 · 2024: 2 · 2023: 2
FWCI
1.24
Type
journal article
Language
en
Open access
Licence
Locations
  • Gels (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
University of Pittsburgh and the Prince Mahidol Award Youth Program
Topics
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
poly(ethylene glycol) diacrylate, mesenchymal stem cells, chondrogenic differentiation, cartilage regeneration, scaffold mechanical strength, glycosaminoglycan deposition, cell migration, cell proliferation, photoinitiators, tissue integration

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