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Cited by 38Microbial bioremediation and biosurfactantsEnvironmental Chemistry and AnalysisSteroid Chemistry and Biochemistry

Enhanced Bioremediation of Aged Polycyclic Aromatic Hydrocarbons in Soil Using Immobilized Microbial Consortia Combined with Strengthening Remediation Strategies

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Zhou, Haixuan, Gao, Xiurong, Wang, Suhang, Zhang, Youchi, Coulon, Frederic, Cai, Chao·2023·International Journal of Environmental Research and Public Health
DOI 10.3390/ijerph20031766PMC PMC9914441
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Abstract

Microbial biodegradation is considered as one of the most effective strategies for the remediation of soil contaminated with polycyclic aromatic hydrocarbons (PAHs). To improve the degradation efficiency of PAHs, PAH-degrading consortia combined with strengthening remediation strategies was used in this study. The PAH biodegrading performance of seven bacterial consortia constructed by different ratios of Mycobacterium gilvum MI, Mycobacterium sp. ZL7 and Rhodococcus rhodochrous Q3 was evaluated in an aqueous system containing phenanthrene, pyrene, benzo[a]pyrene and benzo[b]fluoranthene. Bacterial consortium H6 (Q3:ZL7:MI = 1:2:2) performed a high degrading efficiency of 59% in 8 days. The H6 was subsequently screened to explore its potential ability and performance to degrade aged PAHs in soils from a coking plant and the effects of strengthening strategies on the aged PAH degradation, including the addition of glucose or sodium dodecyl benzene sulfonate (SDBS) individually or as a mixture along immobilization of the inoculant on biochar. The highest degradation efficiencies, which were 15% and 60% for low-molecular-weight (LMW) PAHs and high-molecular-weight (HMW) PAHs, respectively, were observed in the treatment using immobilized microbial consortium H6 combined with the addition of glucose and SDBS after 24 days incubation. This study provides new insights and guidance for future remediation of aged PAH contaminated soils.

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  • The combination of nutrient supplementation and immobilization on biochar significantly improved the degradation of aged PAHs in contaminated soils.

    “This combination significantly improved the degradation of aged PAHs in contaminated soils, making it a viable approach for long-term bioremediation projects [408,409].”

    From Harnessing bacterial consortia for effective bioremediation: targeted removal of heavy metals, hydrocarbons, and persistent pollutants (2025) · cites this paper as [409]

    The Results (section 3.3) report significantly higher degradation of high-molecular-weight PAHs in treatments using a consortium immobilized on biochar; the highest overall degradation occurred when glucose and SDBS were also added. The study lasted 24 days, so it does not establish the citing sentence’s claim that the approach is viable for long-term projects. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

  • Consortia of Mycobacterium, Rhodococcus, and other PAH-degrading bacteria achieved up to 60% degradation of high-molecular-weight PAHs when combined with nutrient supplementation and microbial immobilization on biochar.

    “Similarly, consortia of Mycobacterium, Rhodococcus, and other PAH-degrading bacteria have shown high degradation efficiencies of up to 60% for high-molecular-weight PAHs when combined with strategies such as nutrient supplementation and microbial immobilization on biochar.”

    From Harnessing bacterial consortia for effective bioremediation: targeted removal of heavy metals, hydrocarbons, and persistent pollutants (2025) · cites this paper as [409]

  • Techniques such as metagenomics and proteomics allow for a deeper understanding of microbial communities and their metabolic pathways, enabling the development of strategies to mitigate the production of harmful intermediates.

    “Techniques such as metagenomics and proteomics allow for a deeper understanding of microbial communities and their metabolic pathways, enabling the development of strategies to mitigate the production of harmful intermediates [408,409].”

    From Harnessing bacterial consortia for effective bioremediation: targeted removal of heavy metals, hydrocarbons, and persistent pollutants (2025) · cites this paper as [409]

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Cited by

Sorted by citations

Authors
Zhou, H., Gao, X., Wang, S., Zhang, Y., Coulon, F., & Cai, C.
Affiliations
  • Chinese Academy of Sciences (CN)
  • School of Water, Energy and Environment, Cranfield University, Cranfield MK43 0AL, UK
Corresponding author
Cai, Chao
ORCID
Venue
International Journal of Environmental Research and Public Health
Publisher
MDPI AG
Published
2023-01-18
Volume
20
Issue
3
Pages
1766
ISSN
1660-4601 (ISSN-L)
DOI
10.3390/ijerph20031766
PMC
PMC9914441
OpenAlex
W4317423586
Cited by
38
References
1
Citations by year
2026: 11 · 2025: 9 · 2024: 14 · 2023: 4
FWCI
2.45
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Environmental Research and Public Health (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • CERES (Cranfield University) (submittedVersion, cc-by)
Funders
  • National Key Research and Development Program of China (2018YFC1803300, XDA23030202, 41271324)
  • Strategic Priority Research Program of the Chinese Academy of Sciences, China (2018YFC1803300, XDA23030202, 41271324)
  • National Natural Science Foundation of China (2018YFC1803300, XDA23030202, 41271324)
Topics
  • Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
  • Physical Sciences › Environmental Science › Environmental Chemistry › Environmental Chemistry and Analysis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
Keywords
polycyclic aromatic hydrocarbons, microbial bioremediation, biochar, Mycobacterium gilvum, Rhodococcus rhodochrous, glucose supplementation, sodium dodecyl benzene sulfonate, phenanthrene, pyrene, benzo[a]pyrene, benzo(b)fluoranthene, low molecular weight PAHs, high molecular weight PAHs
MeSH terms
Bacteria, Biodegradation, Environmental, Polycyclic Aromatic Hydrocarbons, Soil, Soil Microbiology, Soil Pollutants, Microbial Consortia

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