Quantification and Purification of Mangiferin from Chinese Mango (Mangifera indica L.) Cultivars and Its Protective Effect on Human Umbilical Vein Endothelial Cells under H2O2-induced Stress
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Mangiferin is a natural xanthonoid with various biological activities. Quantification of mangiferin in fruit peel, pulp, and seed kernel was carried out in 11 Chinese mango (Mangifera indica L.) cultivars. The highest mangiferin content was found in the peel of Lvpimang (LPM) fruit (7.49 mg/g DW). Efficient purification of mangiferin from mango fruit peel was then established for the first time by combination of macroporous HPD100 resin chromatography with optimized high-speed counter-current chromatography (HSCCC). Purified mangiferin was identified by both HPLC and LC-MS, and it showed higher DPPH(•) free-radical scavenging capacities and ferric reducing ability of plasma (FRAP) than by l-ascorbic acid (Vc) or Trolox. In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress. Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress. Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.
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Cell survival increases in H2O2-treated HUVECs when mangiferin (10 and 20 microM) is administered, through its free-radical scavenging ability.
“In addition, cell survival increases in H 2 O 2 -treated HUVECs when mangiferin (10 and 20 µΜ) is administered, through its free-radical scavenging ability.”
From Mangiferin: An effective therapeutic agent against several disorders (Review) (2018) · cites this paper as [93]
Section 2.4 reports increased survival of H2O2-treated HUVECs given mangiferin, but the tested doses were 10 and 20 μg/mL, not 10 and 20 μM. Section 2.3 demonstrates free-radical scavenging in a separate assay; Section 2.4 says the mechanism of the protective effect remains to be investigated, so it does not establish scavenging as the cause of increased cell survival. (LLM, conf=0.91)
Experimental — accuracy not yet measuredA ferric reducing ability of plasma assay demonstrated mangiferin's antioxidant capacity.
“A ferric reducing ability of plasma assay also demonstrated it antioxidant capacity (93).”
From Mangiferin: An effective therapeutic agent against several disorders (Review) (2018) · cites this paper as [93]
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References 30 · Cited by 131
Research works printed but not linked: 20
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- Authors
- Luo, F., Lv, Q., Zhao, Y., Hu, G., Huang, G., Zhang, J., Sun, C., Li, X., & Chen, K.
- Affiliations
- Zhejiang University (CN)
- College of Pharmaceutical Sciences, Zhejiang University, Zijingang Campus, Hangzhou 310058, China
- South China Agricultural University (CN)
- Guangxi Subtropical Crops Research Institute (CN)
- Corresponding author
- Li, Xian
- Venue
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Published
- 2012-09-10
- Volume
- 13
- Issue
- 9
- Pages
- 11260-11274
- ISSN
- 1422-0067 (ISSN-L)
- PMC
- PMC3472743
- OpenAlex
- W1970244964
- Cited by
- 131
- References
- 30
- Citations by year
- 2026: 3 · 2025: 14 · 2024: 15 · 2023: 13 · 2022: 7 · 2021: 15 · 2020: 16 · 2019: 8 · 2018: 4 · 2017: 13 · 2016: 8 · 2015: 8 · 2014: 4 · 2013: 3
- FWCI
- 11.69
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- International Journal of Molecular Sciences (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Higher Education Discipline Innovation Project
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Mangiferin and Mango Extracts
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- mangiferin, mango, xanthones, mango cultivars, fruit peel, high-speed countercurrent chromatography, antioxidant activity, DPPH radical scavenging, ferric reducing antioxidant power, human umbilical vein endothelial cells, H2O2-induced oxidative stress, endothelial protection
- MeSH terms
- China, Chromatography, High Pressure Liquid, Fruit, Humans, Hydrogen Peroxide, Plant Extracts, Free Radical Scavengers, Oxidative Stress, Mangifera, Xanthones, Human Umbilical Vein Endothelial Cells
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