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Cited by 66RNA Research and SplicingRNA and protein synthesis mechanismsMolecular Biology Techniques and Applications

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Impact of Methods on the Measurement of mRNA Turnover

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Wada, Takeo, Becskei, Attila·2017·International Journal of Molecular Sciences
DOI 10.3390/ijms18122723PMC PMC5751324

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Abstract

The turnover of the RNA molecules is determined by the rates of transcription and RNA degradation. Several methods have been developed to study RNA turnover since the beginnings of molecular biology. Here we summarize the main methods to measure RNA half-life: transcription inhibition, gene control, and metabolic labelling. These methods were used to detect the cellular activity of the mRNAs degradation machinery, including the exo-ribonuclease Xrn1 and the exosome. On the other hand, the study of the differential stability of mature RNAs has been hampered by the fact that different methods have often yielded inconsistent results. Recent advances in the systematic comparison of different method variants in yeast have permitted the identification of the least invasive methodologies that reflect half-lives the most faithfully, which is expected to open the way for a consistent quantitative analysis of the determinants of mRNA stability.

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Metadata

Authors
Wada, T., & Becskei, A.
Affiliations
University of Basel (CH)
Corresponding author
Becskei, Attila
ORCID
Becskei, Attila
Venue
International Journal of Molecular Sciences
Publisher
MDPI AG
Published
2017-12-15
Volume
18
Issue
12
Pages
2723
ISSN
1422-0067 (ISSN-L)
DOI
10.3390/ijms18122723
PMC
PMC5751324
OpenAlex
W2767684957
Cited by
66
References
2
Citations by year
2026: 1 · 2025: 4 · 2024: 11 · 2023: 4 · 2022: 21 · 2021: 5 · 2020: 10 · 2019: 7 · 2018: 1 · 2017: 1
FWCI
0.49
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Molecular Sciences (publishedVersion, cc-by)
  • Preprints.org (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • edoc (University of Basel) (acceptedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • edoc (University of Basel)
Funders
  • National Science Foundation
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (152742)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
Keywords
mRNA turnover, RNA half-life, mRNA stability, transcription inhibition, metabolic labeling, exosomes, method comparison, yeast
MeSH terms
Exoribonucleases, RNA, Messenger, Saccharomyces cerevisiae, Gene Expression Regulation, Fungal, RNA Stability, Saccharomyces cerevisiae Proteins, Exosomes, Nonsense Mediated mRNA Decay

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