Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments

Sandomenico, Annamaria, Sivaccumar, Jwala P., Ruvo, Menotti·2020·International Journal of Molecular Sciences

Cited by (134)

Items 101–134 of 134

  1. Expression of monoclonal antibodies for functional and structural studies
    Vashti Irani, Caroline Soliman, Marcus A. Raftis, Andrew J. Guy, Aaron James Elbourne, Paul A. Ramsland · 2022 · Methods in microbiology
  2. Differential scanning fluorimetry as a measure of functionality of refolded anti-malarial antibody fragments
    Ashwathi Valiyaparambil, Deepak K Jagannath, Vysakh K. Viswanath · 2022 · bioRxiv (Cold Spring Harbor Laboratory)
  3. Production and characterization of an Fv-clasp of rheumatoid factor, a low-affinity human autoantibody
    Maruto Yamamoto, Ryoma Funada, Ryota Taki · 2023 · The Journal of Biochemistry
  4. Expression of Synthetic pac Gene Encoding Penicillin G Acylase (PGA) Enzyme in E. coli BL21(DE3) and HB101
    Achmad Makin Amin, Sismindari, Purwanto Purwanto · 2024 · Current Applied Science and Technology
  5. Recent developments in bioprocessing of recombinant antibody fragments
    Nevena Zelenović, Lidija Filipović, Milica Popović · 2023 · Biochemistry (Moscow)
  6. An important consideration when expressing mAbs in Escherichia coli
    William B. Siegall, Rachel B. Lyon, Zvi Kelman · 2024 · Protein Expression and Purification
  7. Enzyme Cascade Amplification-Based Immunoassay Using Alkaline Phosphatase-Linked Single-Chain Variable Fragment Fusion Tracer and MnO2 Nanosheets for Detection of Deoxynivalenol in Corn Samples
    Guifang Xie, Fujing Mao, Yirui Huang · 2024 · Foods
  8. Extending linker sequences between antigen-recognition modules provides more effective production of bispecific nanoantibodies in the periplasma of E. coli
    Sergei V. Tillib, Oksana S. Goryainova · 2024 · Biochemistry (Moscow)
  9. Isolation of anti-Ancylostoma-secreted protein 5 (ASP5) antibody from a naïve antibody phage library
    Brenda Pei Chui Song, Jing Yi Lai, Yee Siew Choong, Nafiseh Khanbabaei, Andreas Latz, Theam Soon Lim · 2024 · Journal of Immunological Methods
  10. Expressão Heteróloga de Inibidores Teciduais de Metaloproteinases (TIMP) com potencial terapêutico em Aspergillus nidulans
    Paula Macedo Cunha · 2024
  11. Novel Bio-Engineering Techniques for Construction of Next-Generation Monoclonal Antibodies in the Framework of Personalized Medicine
    Mahdi Barazesh, Shiva Mohammadi, Sajad Jalili · 2024 · IntechOpen eBooks
  12. Generation of ENO1 Single Chain Variable Fragment and Its Inhibitory effect on cervical cancer cells
    Rui Yang, Yuanyuan Chen, Xiaofeng Liu · 2025 · Research Square
  13. Rapid Antibody Fragment Production and Binding Analysis Using Cell-Free Protein Synthesis Combined with Fluorescence Correlation Spectroscopy
    Shakiba Nikfarjam, Chao Liu, Emma J. Laurence, Adam N. Laurence, Jennifer L. Chlebek, Dante P. Ricci, Steven A. Hoang-Phou, Ted A. Laurence, Matthew A. Coleman · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
  14. Lysis and IB washing procedures significantly impact the recovery of rHumanized ranibizumab from inclusion bodies expressed in Escherichia coli
    Rucha S. Patil, Nida Khan, Anurag Singh Rathore · 2025 · Bioprocess and Biosystems Engineering
  15. Comparative evaluation of E. coli expression systems for soluble hScFv-IL-17A with an unpaired CDR-L3 cysteine
    Weeraya Thongkum, Kanokporn Sornsuwan, Thanathat Pamonsupornwichit · 2025 · Journal of Genetic Engineering and Biotechnology
  16. Additional file 1 of Yields and product comparison between Escherichia coli BL21 and W3110 in industrially relevant conditions: anti-c-Met scFv as a case study
    Klaudia Arauzo‐Aguilera, Luisa Buscajoni, Karin Koch · 2024 · Figshare
  17. Additional file 1 of SHTXTHHly, an extracellular secretion platform for the preparation of bioactive peptides and proteins in Escherichia coli
    Zhu, Wen, Yang Wang, Liangyin Lv · 2022 · Figshare
  18. Heterologous Expression of TIMP Therapeutic Protein in Aspergillus nidulans
    Paula Macedo Cunha · 2024 · LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas)
  19. Introduction
    Naoya Iwamoto · 2026 · Springer theses
  20. Recombinant expression and purification of TANB77-derived pilin-like proteins 935 and 938
    Tham Truong Phuong Tran, Linh Thanh Nguyen, Byeong-Gu Han · 2026 · Protein Expression and Purification
  21. Streamlining bispecific antibody production in E. coli with scFv-Constant domain fusions
    Angela Chiew Wen Ch’ng, Yee Siew Choong, Theam Soon Lim · 2026 · Methods
  22. Optimization, Expression, Purification, and Activity Identification of Anti-PD-1 scFv
    Wan Jiandong, Yu Peining, Liu Yulin · 2026 · Current Protein and Peptide Science
  23. Development of single chain antibodies for targeting Ewing sarcoma surface marker CD99
    Yogalakshmi Sabapathy, Priya Ramanathan, Thangarajan Rajkumar · 2026 · Biochemical and Biophysical Research Communications
  24. Generation and Panning of a Naïve Human scFv Phage Display Library
    Jia Xuan Yeoh, Jing Yi Lai, Angela Chiew Wen Ch’ng · 2026 · Methods in molecular biology
  25. Versatile vector tools for efficient protein screening across multiple expression systems
    Z G Zhu, Yaqing Liu, Lei Qin · 2026 · FEBS Open Bio
  26. An Optimized and Systematically Integrated Expression, Extraction, and Purification Strategy Enabling Efficient Production of TCR-Like Antibodies in Escherichia coli.
    Bassam Ali Sachit, Sylvia Annabel Dass, Rehasri Selva Rajan, Gee Jun Tye, Venugopal Balakrishnan · 2026 · Current Microbiology
  27. A new trastuzumab-ageritin-based immunotoxin that specifically kills breast cancer cells
    Angela Oliver, Nicola Landi, Emanuela Iaccarino, Sara Ragucci, Maria Cantile, Raffaele Ronca, Speranza Annunziata, Giovannina Barisciano, Riccardo Sanna, Paolo V. Pedone et al. · 2026 · International Journal of Biological Macromolecules
  28. From Gene to Protein: Advances and Challenges in Microbial Production of Immunoglobulins
    Xinhui Pang, Xin Song, Yongjun Xia, Guangqiang Wang, X. H. Liu, Zhiqiang Xiong, Lianzhong Ai · 2026 · Fermentation
  29. Multimodal chromatography solutions for the removal of light chain impurity from a fab therapeutic
    Dongyoun Jang, Nathan Nicholes, Steven M. Cramer · 2026 · Biotechnology Progress
  30. Rapid cell-free antibody fragment production and binding analysis via fluorescence correlation
    Shakiba Nikfarjam, Chao Liu, Emma J. Laurence, Adam Laurence, Jennifer L. Chlebek, Dante P. Ricci, Steven A. Hoang-Phou, Isabella M. Carrano, Ted A. Laurence, Matthew A. Coleman · 2026 · Methods
  31. Recombinant AflQ: a novel fungal-derived protein with potent antibacterial properties
    Marwah M. Ohmayed, Dhafer Fakhri Al-Rawi, Harith K. Buniya · 2026 · Biotechnology Letters
  32. Downstream Processing and Innovative Purification Strategies for Large-Scale Recovery of Pure Hydrophobins
    Rahul Kumar, Mohammad Aves, Shraddha Bhaskar Sawant · 2026 · IGI Global eBooks
  33. Advances and applications of engineered protein therapeutics and their role in precision medicine
    Wadha Aldossary, Nada Alqahtani, Layla Asiri, Noora Alkhurayef, Samar Alagl, Sana Alturki, Duha Saad, Mashael Al Yahya, Samah Al-Harthi · 2026 · Frontiers in Bioengineering and Biotechnology
  34. A self-assembled and covalently stabilized FcRn-binding albumin fragment-payload assembly.
    Yong Joon Cho, Gyu-Jin Lee, Jong-Ha Park, Se-Jin Lim, Seung-Min Shin, Young-Jun An, Sun Oh Shin, Sung-In Lim · 2026 · International Journal of Biological Macromolecules