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Cited by 73D Printing in Biomedical ResearchCellular Mechanics and InteractionsMicrofluidic and Bio-sensing Technologies

Evaluation of Lipid Accumulation Using Electrical Impedance Measurement under Three-Dimensional Culture Condition

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Zemmyo, Daiki, Miyata, Shogo·2019·Micromachines
DOI 10.3390/mi10070455PMC PMC6680657
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Abstract

The degeneration of adipocyte has been reported to cause obesity, metabolic syndrome, and other diseases. To treat these diseases, an effective in vitro evaluation and drug-screening system for adipocyte culture is required. The objective of this study is to establish an in vitro three-dimensional cell culture system to enable the monitoring of lipid accumulation by measuring electrical impedance, and to determine the relationship between the impedance and lipid accumulation of adipocytes cultured three dimensionally. Consequently, pre-adipocytes, 3T3-L1 cells, were cultured and differentiated to the adipocytes in our culture system, and the electrical impedance of the three-dimensional adipocyte culture at a high frequency was related to the lipid accumulation of the adipocytes. In conclusion, the lipid accumulation of adipocytes could be evaluated in real time by monitoring the electrical impedance during in vitro culture.

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Cited by

Sorted by citations

Authors
Zemmyo, D., & Miyata, S.
Affiliations
Keio University (JP)
Corresponding author
Miyata, Shogo
ORCID
Miyata, Shogo
Venue
Micromachines
Publisher
MDPI AG
Published
2019-07-06
Volume
10
Issue
7
Pages
455
ISSN
2072-666X (ISSN-L)
DOI
10.3390/mi10070455
PMC
PMC6680657
OpenAlex
W2955749058
Cited by
7
References
0
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2020: 1 · 2019: 1
FWCI
0.18
Type
journal article
Language
en
Open access
Licence
Locations
  • Micromachines (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Japan Agency for Medical Research and Development
  • Japan Society for the Promotion of Science (26560222, 17K01369 and 26560222, 17K01369)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
Keywords
lipid accumulation, electrical impedance measurement, 3D cell culture, adipocytes, 3T3-L1 cells, adipogenesis, preadipocytes, in vitro drug testing, obesity, metabolic syndrome

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