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Translational Frameshifting in the chlD Gene Gives a Clue to the Coevolution of the Chlorophyll and Cobalamin Biosyntheses
Open accessAbstract
Today, hundreds of prokaryotic species are able to synthesize chlorophyll and cobalamin (vitamin B12). An important step in the biosynthesis of these coenzymes is the insertion of a metal ion into a porphyrin ring. Namely, Mg-chelatase ChlIDH and aerobic Co-chelatase CobNST are utilized in the chlorophyll and vitamin B12 pathways, respectively. The corresponding subunits of these enzymes have common evolutionary origin. Recently, we have identified a highly conserved frameshifting signal in the chlD gene. This unusual regulatory mechanism allowed production of both the small and the medium chelatase subunits from the same gene. Moreover, the chlD gene appeared early in the evolution and could be at the starting point in the development of the chlorophyll and B12 pathways. Here, we studied the possible coevolution of these two pathways through the analysis of the chelatase genes. To do that, we developed a specialized Web database with comprehensive information about more than 1200 prokaryotic genomes. Further analysis allowed us to split the coevolution of the chlorophyll and B12 pathway into eight distinct stages.
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“Earlier gene‐based investigations of corrin biosynthesis evolution [80, 155, 156, 157] have missed the role of corrinoids in CoFeS and the acetyl‐CoA pathway, even though their function in ancient pathways has long been evident [7, 8, 48, 49].”
From Evidence for corrin biosynthesis in the last universal common ancestor (2025) · cites this paper as [157]
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Cited by
Sorted by citations
Evidence for corrin biosynthesis in the last universal common ancestor
Modjewski, L. D., Karavaeva, V., Mrnjavac, N., Knopp, M., Martin, W. F., & Sousa, F. L. (2025) · The FEBS Journal · doi:10.1111/febs.17367 · Cited by 15
“Earlier gene-based investigations of corrin biosynthesis evolution [80,155 – 157] have missed the role of corri-noids in CoFeS and the acetyl-CoA pathway, even though their function in ancient pathways has long been evident [7,8,48,49].”
Metadata
- Authors
- Kuznetsov, S., Milenkin, A., & Antonov, I.
- Affiliations
- Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia
- Moscow Institute of Physics and Technology (RU)
- National Research University Higher School of Economics (RU)
- Corresponding author
- Antonov, Ivan
- ORCID
- Antonov, Ivan
- Venue
- Microorganisms
- Publisher
- MDPI AG
- Published
- 2022-06-11
- Volume
- 10
- Issue
- 6
- Pages
- 1200
- ISSN
- 2076-2607 (ISSN-L)
- PMC
- PMC9227772
- OpenAlex
- W4282600783
- Cited by
- 3
- References
- 1
- Citations by year
- 2024: 2 · 2023: 1
- FWCI
- 0.24
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Microorganisms (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Russian Science Foundation (20-74-00128)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Porphyrin Metabolism and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Physical Sciences › Materials Science › Materials Chemistry › Porphyrin and Phthalocyanine Chemistry
- Keywords
- translational frameshifting, chlorophyll biosynthesis, vitamin B12 biosynthesis, vitamin B12, Mg-chelatase, coevolution, prokaryotic genomes, gene regulatory mechanisms
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