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Cited by 45Microbial bioremediation and biosurfactantsMicrobial Natural Products and BiosynthesisSurfactants and Colloidal Systems

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Effects of Different Cultivation Parameters on the Production of Surfactin Variants by a Bacillus subtilis Strain

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Bartal, Attila, Vigneshwari, Aruna, Bóka, Bettina, Vörös, Mónika, Takács, István, Kredics, László, Manczinger, László, Varga, Mónika, Vágvölgyi, Csaba, Szekeres, András·2018·Molecules
DOI 10.3390/molecules23102675PMC PMC6222309
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Abstract

Surfactins are lipopeptide-type biosurfactants produced mainly by Bacillus species, consisting of a peptide loop of seven amino acids and a hydrophobic fatty acid chain (C12–C16). These molecules have been proven to exhibit various biological activities; thus, their therapeutic and environmental applications are considered. Within the surfactin lipopeptide family, there is a wide spectrum of different homologues and isomers; to date, more than 30 variants have been described. Since the newest members of these lipopeptides were described recently, there is no information that is available on their characteristic features, e.g., the dependence of their production from different cultivation parameters. This study examined the effects of both the different carbon sources and various metal ions on the surfactin production of a selected B. subtilis strain. Among the applied carbon sources, fructose and xylose had the highest impacts on the ratio of the different variants, regarding both the peptide sequences and the lengths of the fatty acids. Furthermore, the application of metal ions Mn2+, Cu2+ and Ni2+ in the media completely changed the surfactin variant compositions of the fermenting broths leading to the appearance of methyl esterified surfactin forms, and resulted in the appearance of novel surfactin variants with fatty acid chains containing no more than 11 carbon atoms.

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  • The biosynthesis and properties of surfactants are determined by the genetic characteristics of the microorganism strain, the composition of the medium, and the growth conditions.

    “The biosynthesis and properties of surfactants are determined by the genetic characteristics of the microorganism strain, the composition of the medium, and the growth conditions [28,37].”

    From Influence of the Medium Composition and the Culture Conditions on Surfactin Biosynthesis by a Native Bacillus subtilis natto BS19 Strain (2021) · cites this paper as [37]

    The Introduction says genetically encoded differences, medium composition, and cultivation parameters affect surfactin production and variant profiles. The Abstract and Conclusions report changes in variant composition under different culture conditions. The paper does not establish that these factors determine surfactant properties generally, so the claim extends beyond its evidence on surfactins. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • Biosynthesis of the desired surfactin analog depends on both the properties of the strain and the culture conditions.

    “Thus, biosynthesis of the desired surfactin analog depends on both the properties of the strain and the culture conditions.”

    From Influence of the Medium Composition and the Culture Conditions on Surfactin Biosynthesis by a Native Bacillus subtilis natto BS19 Strain (2021) · cites this paper as Bartal et al. [37]

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Metadata

Authors
Bartal, A., Vigneshwari, A., Bóka, B., Vörös, M., Takács, I., Kredics, L., Manczinger, L., Varga, M., Vágvölgyi, C., & Szekeres, A.
Affiliations
University of Szeged (HU)
Corresponding author
Szekeres, András
ORCID
Venue
Molecules
Publisher
MDPI AG
Published
2018-10-18
Volume
23
Issue
10
Pages
2675
ISSN
1420-3049 (ISSN-L)
DOI
10.3390/molecules23102675
PMC
PMC6222309
OpenAlex
W2897490421
Cited by
45
References
1
Citations by year
2026: 5 · 2025: 4 · 2024: 3 · 2023: 8 · 2022: 7 · 2021: 10 · 2020: 5 · 2019: 2 · 2018: 1
FWCI
1.69
Type
journal article
Language
en
Open access
Licence
Locations
  • Molecules (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • SZTE Publicatio Repozitórium (University of Szeged) (acceptedVersion, other-oa)
  • Repository of the Academy's Library (Library of the Hungarian Academy of Sciences) (acceptedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Hungarian Government and the European Union (GINOP-2.3.2-15-2016-00012)
  • Hungarian Scientific Research Fund (OTKA K-128659)
  • János Bolyai Research Scholarship (Hungarian Academy of Sciences) (KL)
Topics
  • Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Microbial Natural Products and Biosynthesis
  • Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Surfactants and Colloidal Systems
Keywords
surfactin, Bacillus subtilis, lipopeptide biosurfactant, carbon source, fructose, xylose, metal ions, manganese, copper, nickel, fatty acid chain length
MeSH terms
Amino Acid Sequence, Bacillus subtilis, Carbon, Isomerism, Peptides, Cyclic, Surface-Active Agents, Lipopeptides

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