Walnut Oil Prevents Scopolamine-Induced Memory Dysfunction in a Mouse Model
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For thousands of years, it has been widely believed that walnut is a kind of nut that has benefits for the human body. Walnut oil, accounting for about 70% of walnut, mainly consists of polyunsaturated fatty acids. To investigate the effect of walnut oil on memory impairment in mice, scopolamine (3 mg/kg body weight/d) was used to establish the animal model during Morris Water Maze (MWM) tests. Walnut oil was administrated orally at 10 mL/kg body weight/d for 8 consecutive weeks. The results showed that walnut oil treatment ameliorated the behavior of the memory-impaired mice in the MWM test. Additionally, walnut oil obviously inhibited acetylcholinesterase activity (1.26 ± 0.12 U/mg prot) (p = 0.013) and increased choline acetyltransferase activity (129.75 ± 6.76 U/mg tissue wet weight) in the brains of scopolamine-treated mice (p = 0.024), suggesting that walnut oil could prevent cholinergic function damage in mice brains. Furthermore, walnut oil remarkably prevented the decrease in total superoxide dismutase activity (93.30 ± 5.50 U/mg prot) (p = 0.006) and glutathione content (110.45 ± 17.70 mg/g prot) (p = 0.047) and the increase of malondialdehyde content (13.79 ± 0.96 nmol/mg prot) (p = 0.001) in the brain of scopolamine-treated mice, indicating that walnut oil could inhibit oxidative stress in the brain of mice. Furthermore, walnut oil prevented histological changes of neurons in hippocampal CA1 and CA3 regions induced by scopolamine. These findings indicate that walnut oil could prevent memory impairment in mice, which might be a potential way for the prevention of memory dysfunctions.
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Sapindus laurifolia and walnut oil significantly reduced oxidative stress and AChE levels (p<0.001).
“Sapindus laurifolia and walnut oil significantly reduced oxidative stress and AChE levels (p < 0.001) [36,37].”
From Cholinesterase Inhibitors from Plants and Their Potential in Alzheimer’s Treatment: Systematic Review (2025) · cites this paper as [37]
Results §2.2 reports that walnut oil reduced brain AChE activity in scopolamine-treated mice, and Results §2.3 reports improved oxidative-stress measures. The claimed p < 0.001 does not match the reported AChE result (p = 0.013) or the reported antioxidant measures; this paper does not study Sapindus laurifolia. The sentence has two citations, so the Sapindus finding is not necessarily attributed to this source. (LLM, conf=0.90)
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References 45 · Cited by 53
- Authors
- Liao, J., Nai, Y., Feng, L., Chen, Y., Li, M., & Xu, H.
- Affiliations
- Northwest A&F University (CN)
- College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
- Corresponding author
- Li, Mei
- Xu, Huaide
- Venue
- Molecules
- Publisher
- MDPI AG
- Published
- 2020-04-02
- Volume
- 25
- Issue
- 7
- Pages
- 1630
- ISSN
- 1420-3049 (ISSN-L)
- PMC
- PMC7180932
- OpenAlex
- W3014924473
- Cited by
- 53
- References
- 45
- Citations by year
- 2026: 3 · 2025: 10 · 2024: 9 · 2023: 16 · 2022: 9 · 2021: 6
- FWCI
- 8.87
- Type
- journal article
- Language
- en
- Open access
- gold
- dx.doi.org
- Licence
- Locations
- Molecules (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Pharmacology › Cholinesterase and Neurodegenerative Diseases
- Health Sciences › Nursing › Nutrition and Dietetics › Nuts composition and effects
- Keywords
- walnut oil, scopolamine-induced amnesia, memory impairment, acetylcholinesterase inhibition, choline acetyltransferase, cholinergic activity, oxidative stress, antioxidant enzymes, hippocampal neurons, neuroprotection, polyunsaturated fatty acids
- MeSH terms
- Administration, Oral, Animals, Brain, Choline O-Acetyltransferase, Disease Models, Animal, Memory Disorders, Plant Oils, Scopolamine, Gene Expression Regulation, Enzymologic, Oxidative Stress, Maze Learning, Juglans, Mice
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