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Cited by 31Oral microbiology and periodontitis researchDental materials and restorationsDental Implant Techniques and Outcomes

Cytomorphometric Analysis of Inflammation Dynamics in the Periodontium Following the Use of Fixed Dental Prostheses

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Heboyan, Artak, Syed, Azeem Ul Yaqin, Rokaya, Dinesh, Cooper, Paul R., Manrikyan, Mikael, Markaryan, Marina·2020·Molecules
DOI 10.3390/molecules25204650PMC PMC7587339
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Abstract

Cytomorphometry is used in the sampling of biological materials and diagnostic procedures. The use of cytological studies in periodontal diseases is not well described in the literature. Our study aimed to quantitatively assess the inflammation dynamics using cytomorphometric analysis of the periodontium before and after the use of fixed dental prostheses. Following ethics approval, a total of 105 subjects were divided in 3 groups as gingivitis (n = 23), periodontitis (n = 58), and healthy periodontium (control) (n = 24). The fixed dental prostheses (crowns and fixed partial dentures) were fabricated from cobalt-chrome metal-ceramic prostheses using the conventional method (C/M-CoCr), cobalt-chrome metal-ceramic prostheses by the computer-aided design and computer-aided manufacturing (CAD/CAM) technique (C/C-CoCr), and zirconia-based ceramic prostheses by the CAD/CAM technique (C/C-Zr) among subjects with gingivitis and periodontitis. The gingival crevicular fluid (GCF) was obtained from subjects before and after the use of the prostheses. The total count of epithelial cells and the connective tissue cells or polymorphonuclear neutrophils (PMNs) in GCF were studied using cytomorphometric analysis. The Statistical Package Tor the Social Sciences (SPSS), Version 20 (IBM Company, Chicago, IL, USA) was used to analyze the results and the significance level was set at p = 0.05. The data for before and after the use of the prostheses were compared using independent t-Tests. Similarly, the results after the use of prostheses in gingivitis, periodontitis, and control in each type of prostheses were compared using One-way ANOVA with post hoc using Scheffe. The total epithelial cells and the PMNs were determined along with the epithelium/leukocyte index. Regardless of the prostheses type used, no significant change in the parameters was identified among patients with a healthy periodontium, before and after prosthetic treatment. In all study groups, a statistically increase (p value < 0.05) was observed in the oral epithelial cell counts and a statistically decrease (p < 0.05) in the PMNs count following the use of the fixed prostheses. Data on cytomorphometric analysis could enable the selection of the most appropriate prostheses for use in patients with periodontal pathologies. When choosing prostheses, changes in the composition of GCF could be considered as a useful criterion for their use.

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  • Cytomorphometric analysis before and after insertion of fixed Cr-Co metal-ceramic prostheses fabricated by conventional method and CAD/CAM, as well as zirconia prostheses fabricated by CAD/CAM, has shown an increase in oral epithelial cell count and a decrease in PMN count.

    “the cytomorphometric analysis of the periodontium before and after the insertion of fixed Cr-Co metal-ceramic prostheses fabricated by the conventional method and the CAD/CAM system, as well as the use of zirconia prostheses fabricated by the latter technique has shown an increase in the oral epithelial cell count and a decrease in the PMN count.”

    From Potential Impact of Prosthetic Biomaterials on the Periodontium: A Comprehensive Review (2023) · cites this paper as [61]

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Authors
Heboyan, A., Syed, A. U. Y., Rokaya, D., Cooper, P. R., Manrikyan, M., & Markaryan, M.
Affiliations
  • Yerevan State Medical University (AM)
  • King Faisal University (SA)
  • Walailak University (TH)
  • University of Otago (NZ)
  • Department of Pediatric Dentistry and Orthodontics, Faculty of Stomatology, Yerevan State Medical University, Str. Koryun 2, Yerevan 0025, Armenia
Corresponding author
  • Heboyan, Artak
  • Syed, Azeem Ul Yaqin
  • Rokaya, Dinesh
ORCID
Venue
Molecules
Publisher
MDPI AG
Published
2020-10-12
Volume
25
Issue
20
Pages
4650
ISSN
1420-3049 (ISSN-L)
DOI
10.3390/molecules25204650
PMC
PMC7587339
OpenAlex
W3092275961
Cited by
31
References
40
Citations by year
2025: 1 · 2024: 2 · 2023: 7 · 2022: 17 · 2021: 4
FWCI
7.11
Type
journal article
Language
en
Open access
Licence
Locations
  • Molecules (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
  • Health Sciences › Dentistry › Orthodontics › Dental materials and restorations
  • Health Sciences › Dentistry › Oral Surgery › Dental Implant Techniques and Outcomes
Keywords
periodontitis, gingivitis, fixed partial dentures, gingival crevicular fluid, cytomorphometry, neutrophils, epithelial cells, connective tissue cells
MeSH terms
Adult, Aged, Epithelial Cells, Gingival Crevicular Fluid, Gingivitis, Humans, Inflammation, Middle Aged, Neutrophils, Periodontitis, Periodontium, Dental Prosthesis

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