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Cited by 51Plasma Applications and DiagnosticsMicrobial Inactivation MethodsPhotodynamic Therapy Research Studies

Direct and Indirect Bactericidal Effects of Cold Atmospheric-Pressure Microplasma and Plasma Jet

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Yahaya, Ahmad Guji, Okuyama, Tomohiro, Kristof, Jaroslav, Blajan, Marius Gabriel, Shimizu, Kazuo·2021·Molecules
DOI 10.3390/molecules26092523PMC PMC8123442
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Abstract

The direct and indirect bactericidal effects of dielectric barrier discharge (DBD) cold atmospheric-pressure microplasma in an air and plasma jet generated in an argon-oxygen gas mixture was investigated on Staphylococcus aureus and Cutibacterium acnes. An AC power supply was used to generate plasma at relatively low discharge voltages (0.9–2.4 kV) and frequency (27–30 kHz). Cultured bacteria were cultivated at a serial dilution of 10−5, then exposed to direct microplasma treatment and indirect treatment through plasma-activated water (PAW). The obtained results revealed that these methods of bacterial inactivation showed a 2 and 1 log reduction in the number of survived CFU/mL with direct treatment being the most effective means of treatment at just 3 min using air. UV–Vis spectroscopy confirmed that an increase in treatment time at 1.2% O2, 98.8% Ar caused a decrease in O2 concentration in the water as well as a decrease in absorbance of the peaks at 210 nm, which are attributed NO2− and NO3− concentration in the water, termed denitratification and denitritification in the treated water, respectively.

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  • The antimicrobial effects of CP, involving reactive species attacking bacterial cells, damaging cell walls, increasing membrane permeability, leading to cell membrane rupture, damage to organelles, proteins, and nucleic acids, and microbial cell death, may explain the shifts in genera.

    “The underlying mechanism for these shifts may involve the antimicrobial effects of CP: reactive species generated during CP treatment can attack bacterial cells, damaging cell walls to increase membrane permeability. This process ultimately leads to cell membrane rupture, followed by damage to organelles, proteins, and nucleic acids, culminating in microbial cell death [55,56].”

    From Effect of Atmospheric Cold Plasma Treatment on the Microorganism Growth, Diversity, and Quality of Coconut Water During Refrigerator Storage (2025) · cites this paper as [56]

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Cited by

Sorted by citations

Authors
Yahaya, A. G., Okuyama, T., Kristof, J., Blajan, M. G., & Shimizu, K.
Affiliations
  • Shizuoka University (JP)
  • Organization for Innovation and Social Collaboration, Shizuoka University, Hamamatsu 432-8561, Japan
Corresponding author
Yahaya, Ahmad Guji
ORCID
Venue
Molecules
Publisher
MDPI AG
Published
2021-04-26
Volume
26
Issue
9
Pages
2523
ISSN
1420-3049 (ISSN-L)
DOI
10.3390/molecules26092523
PMC
PMC8123442
OpenAlex
W3159121559
Cited by
51
References
0
Citations by year
2026: 3 · 2025: 18 · 2024: 10 · 2023: 9 · 2022: 10 · 2021: 1
FWCI
3.96
Type
journal article
Language
en
Open access
Licence
Locations
  • Molecules (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Shizuoka University
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Plasma Applications and Diagnostics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
Keywords
plasma jet, dielectric barrier discharge, bactericidal activity, Staphylococcus aureus, Propionibacterium acnes, plasma-activated water, argon-oxygen mixtures, nitrate reduction, nitrite reduction
MeSH terms
Anti-Bacterial Agents, Atmospheric Pressure, Microbial Sensitivity Tests, Ozone, Staphylococcus aureus, Sterilization, Water, Reactive Oxygen Species, Reactive Nitrogen Species, Plasma Gases

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