Quantifying Microalgae Growth by the Optical Detection of Glucose in the NIR Waveband
Open accessAbstract
Microalgae have become a popular area of research over the past few decades due to their enormous benefits to various sectors, such as pharmaceuticals, biofuels, and food and feed. Nevertheless, the benefits of microalgae cannot be fully exploited without the optimization of their upstream production. The growth of microalgae is commonly measured based on the optical density of the sample. However, the presence of debris in the culture and the optical absorption of the intercellular components affect the accuracy of this measurement. As a solution, this paper introduces the direct optical detection of glucose molecules at 940–960 nm to accurately measure the growth of microalgae. In addition, this paper also discusses the effects of the presence of glucose on the absorption of free water molecules in the culture. The potential of the optical detection of glucose as a complement to the commonly used optical density measurement at 680 nm is discussed in this paper. Lastly, a few recommendations for future works are presented to further verify the credibility of glucose detection for the accurate determination of microalgae’s growth.
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NIR and MIR spectroscopic sensors can detect multiple compounds simultaneously.
“In addition, NIR and MIR spectroscopic sensors can detect multiple compounds simultaneously [226].”
From Multi-Sensing Monitoring of the Microalgae Biomass Cultivation Systems for Biofuels and Added Value Products Synthesis—Challenges and Opportunities (2025) · cites this paper as [226]
The Introduction (page unavailable) says spectroscopy has been used to detect several microalgal components, and the Results and Discussion distinguishes NIR absorption wavelengths for glucose and glycolipids. But the paper does not establish that NIR or MIR sensors detect multiple compounds simultaneously; its own measurements focus on glucose. (LLM, conf=0.78)
Experimental — accuracy not yet measuredOptical density (OD) has been the method of choice for the determination of growth and estimation of biomass in cultures of various microalgae.
“Optical density (OD) has been the method of choice for the determination of growth and estimation of biomass in cultures of various microalgae (Thiviyanathan et al. 2023).”
From Quantification of phototrophically grown Galdieria sulphuraria and other microalgae using diphenylamine (2025) · cites this paper as Thiviyanathan et al. 2023
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References 84 · Cited by 19
- Authors
- Thiviyanathan, V. A., Ker, P. J., Amin, E. P. P., Tang, S. G. H., Yee, W., & Jamaludin, M. Z.
- Affiliations
- Universiti Tenaga Nasional (MY)
- National University of Malaysia (MY)
- Universiti Malaysia Terengganu (MY)
- Corresponding author
- Ker, Pin Jern
- Tang, Shirley Gee Hoon
- Venue
- Molecules
- Publisher
- MDPI AG
- Published
- 2023-01-30
- Volume
- 28
- Issue
- 3
- Pages
- 1318
- ISSN
- 1420-3049 (ISSN-L)
- PMC
- PMC9921349
- OpenAlex
- W4318478116
- Cited by
- 19
- References
- 84
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 4 · 2023: 4
- FWCI
- 0.91
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Molecules (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Yayasan Canselor UNITEN Community Project (202210049YCU, J510050002, IC-6 BOL-163 DREFRESH2025)
- UNITEN Bold Refresh Publication Fund 2022 (202210049YCU, J510050002, IC-6 BOL-163 DREFRESH2025)
- Centre of Excellence (202210049YCU, J510050002, IC-6 BOL-163 DREFRESH2025)
- UUniversiti Kebangsaan Malaysia (202210049YCU, J510050002, IC-6 BOL-163 DREFRESH2025)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Physical Sciences › Environmental Science › Waste Management and Disposal › Water Quality Monitoring and Analysis
- Keywords
- microalgae growth, glucose detection, near-infrared spectroscopy, optical density measurement, biofuel production, microalgae cultivation, water absorption, biomass estimation
- MeSH terms
- Food, Biomass, Biofuels, Microalgae
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