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Cited by 56Enzyme Production and CharacterizationEnzyme Catalysis and ImmobilizationBiofuel production and bioconversion

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Recent Advances in β-Glucosidase Sequence and Structure Engineering: A Brief Review

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Ouyang, Bei, Wang, Guoping, Zhang, Nian, Zuo, Jiali, Huang, Yunhong, Zhao, Xihua·2023·Molecules
DOI 10.3390/molecules28134990PMC PMC10343665

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Abstract

β-glucosidases (BGLs) play a crucial role in the degradation of lignocellulosic biomass as well as in industrial applications such as pharmaceuticals, foods, and flavors. However, the application of BGLs has been largely hindered by issues such as low enzyme activity, product inhibition, low stability, etc. Many approaches have been developed to engineer BGLs to improve these enzymatic characteristics to facilitate industrial production. In this article, we review the recent advances in BGL engineering in the field, including the efforts from our laboratory. We summarize and discuss the BGL engineering studies according to the targeted functions as well as the specific strategies used for BGL engineering.

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Metadata

Authors
Ouyang, B., Wang, G., Zhang, N., Zuo, J., Huang, Y., & Zhao, X.
Affiliations
Jiangxi Normal University (CN)
Corresponding author
  • Huang, Yunhong
  • Zhao, Xihua
ORCID
Venue
Molecules
Publisher
MDPI AG
Published
2023-06-25
Volume
28
Issue
13
Pages
4990
ISSN
1420-3049 (ISSN-L)
DOI
10.3390/molecules28134990
PMC
PMC10343665
OpenAlex
W4382060579
Cited by
56
References
2
Citations by year
2026: 18 · 2025: 18 · 2024: 17 · 2023: 3
FWCI
1.67
Type
journal article
Language
en
Open access
Licence
Locations
  • Molecules (publishedVersion, cc-by)
  • Preprints.org (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (22068014, 21666010, 5451)
  • Doctoral Starting up Foundation of Jiangxi Normal University (22068014, 21666010, 5451)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
  • Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
Keywords
β-glucosidase, enzyme engineering, lignocellulose degradation, enzyme stability, product inhibition, enzyme activity enhancement, industrial enzymes, sequence engineering, structural engineering
MeSH terms
beta-Glucosidase

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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