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Cited by 5Liver Disease Diagnosis and TreatmentPhytochemicals and Antioxidant ActivitiesDrug-Induced Hepatotoxicity and Protection

Brassica oleracea Var italica by-Products Prevent Lipid Accumulation and Cell Death in a Liver Cell Model of Lipid Toxicity

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Castelão-Baptista, José P., Valente, Sara A., Canário, Sara, Oppolzer, David, Barros, Ana, Venâncio, Carlos, Martins, Tânia, Antunes, Luís +3 more·2023·Nutrients
DOI 10.3390/nu15040924PMC PMC9965884
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Abstract

Obesity, a rising concern in the Eastern world, encompasses several co-morbidities, namely non-alcoholic fatty liver disease (NAFLD). Potential natural-based interventions to decrease the burden of obesity complications are being investigated. Many of the edible parts of plants are not sold for consumption and end up as massive waste, losing nutritional potential. In fact, a sizeable amount of waste is generated within the different steps of the food supply chain, representing a massive loss of both plant material and natural resources. A good example is Brassica by-products (BBPs). The objective of this work was to investigate the effect of three different extracts from broccoli (Brassica oleracea var italica) by-products in an in vitro model of free fatty acid (FFA)-induced lipotoxicity using human hepatoma HepG2 cells. Broccoli leaf, stalk, and inflorescence extracts induced a dose-dependent decrease in the cell viability of HepG2 cells. However, the maximal non-lethal concentrations of leaves, stalks, and inflorescences (10 μg/mL) did not compromise mitochondrial function or neutral lipid accumulation in HepG2 cells. The extracts significantly decreased FFA-induced lipid accumulation in HepG2 cells either in a co-incubation or pre-incubation strategy. The broccoli extracts’ capacity to prevent the FFA-induced decrease in catalase activity in HepG2 may explain the observed effects.

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  • To induce steatosis in HepG2, supraphysiological concentrations of FFAs (0.5-2.0 mM) are often used.

    “Second, to induce steatosis, supraphysiological concentrations of FFAs (concentrations of 0.5–2.0 mM) are often used in HepG2 experiments [28,91,125,126,127,128,129,130].”

    From The HepG2 Cell Line as a Model for Studying Metabolic Dysfunction-Associated Steatotic Liver Disease (2026) · cites this paper as [129]

    Materials and Methods reports using FFAs to treat HepG2 cells, and Results §3.5 calls the 250 µM dose supraphysiological and reports increased intracellular lipid content. The paper supports the use of supraphysiological FFAs to induce lipid accumulation in HepG2, but its treatment dose is below 0.5–2.0 mM and the cited passages do not establish that range as often used. (LLM, conf=0.86)

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References 51 · Cited by 5

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Authors
Castelão-Baptista, J. P., Valente, S. A., Canário, S., Oppolzer, D., Barros, A., Venâncio, C., Martins, T., Antunes, L., Sardão, V. A., Rosa, E., & Oliveira, P. J.
Affiliations
  • University of Coimbra (PT)
  • University of Trás-os-Montes and Alto Douro (PT)
Corresponding author
Oliveira, Paulo J.
ORCID
Venue
Nutrients
Publisher
MDPI AG
Published
2023-02-12
Volume
15
Issue
4
Pages
924
ISSN
2072-6643 (ISSN-L)
DOI
10.3390/nu15040924
PMC
PMC9965884
OpenAlex
W4320491412
Cited by
5
References
51
Citations by year
2026: 3 · 2025: 1 · 2023: 1
FWCI
1.00
Type
journal article
Language
en
Open access
Licence
Locations
  • Nutrients (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, cc-by)
Funders
  • European Regional Development Fund (ERDF) (CENTRO-01-0246-FEDER-000010, PTDC/ASP-HOR/29152/2017, POCI-01-0145-FEDER-029152, UIDB/04033/2020, UIDB/04539/2020)
  • Fundação para a Ciência e a Tecnologia (CENTRO-01-0246-FEDER-000010, PTDC/ASP-HOR/29152/2017, POCI-01-0145-FEDER-029152, UIDB/04033/2020, UIDB/04539/2020)
  • Portuguese Foundation for Science and Technology (FCT) (CENTRO-01-0246-FEDER-000010, PTDC/ASP-HOR/29152/2017, POCI-01-0145-FEDER-029152, UIDB/04033/2020, UIDB/04539/2020)
  • Directorate General for Higher Education (DGES) (CENTRO-01-0246-FEDER-000010, PTDC/ASP-HOR/29152/2017, POCI-01-0145-FEDER-029152, UIDB/04033/2020, UIDB/04539/2020)
Topics
  • Health Sciences › Medicine › Epidemiology › Liver Disease Diagnosis and Treatment
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
Keywords
broccoli by-products, broccoli, non-alcoholic fatty liver disease, lipid accumulation, HepG2 cells, food waste valorization, catalase activity, oxidative stress, obesity, plant extracts
MeSH terms
Brassica, Humans, Lipids, Liver Neoplasms, Obesity, Cell Death, Hep G2 Cells

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