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Cited by 9Postharvest Quality and Shelf Life ManagementBerry genetics and cultivation researchPlant biochemistry and biosynthesis

Comparative Characterization of Fruit Volatiles and Volatile-Related Genes Expression of ‘Benihoppe’ Strawberry and Its Somaclonal Mutant

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Zhang, Zhuo, Yu, Shuang, Zhang, Zhihong, Zhang, Junxiang, Li, He·2023·Plants
DOI 10.3390/plants12051109PMC PMC10005569
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Abstract

Somaclonal variations in tissue cultures can be used in plant breeding programs. However, it is still unclear whether somaclonal variations and their original parent have differences in volatile compounds, and the candidate genes which result in the differences in volatile compounds also need to be identified. In this study, we utilized the ‘Benihoppe’ strawberry and its somaclonal mutant ‘Xiaobai’, which has different fruit aromas compared with ‘Benihoppe’, as research materials. Using HS-SPME-GC-MS, 113 volatile compounds have been identified in the four developmental periods of ‘Benihoppe’ and ‘Xiaobai’. Among them, the quantity and content of some unique esters in ‘Xiaobai’ were much higher than that in ‘Benihoppe’. In addition, we found that the contents and odor activity values of ethyl isovalerate, ethyl hexanoate, ethyl butyrate, ethyl pentanoate, linalool, and nerolidol in the red fruit of ‘Xiaobai’ were much higher compared with ‘Benihoppe’, which may result from the significantly increased expression of FaLOX6, FaHPL, FaADH, FaAAT, FaAAT1, FaDXS, FaMCS, and FaHDR in ‘Xiaobai’. However, the content of eugenol in ‘Benihoppe’ was higher than that in ‘Xiaobai’, which may result from the higher expression of FaEGS1a in ‘Benihoppe’ compared with ‘Xiaobai’. The results provide insights into the somaclonal variations that affect the volatile compounds in strawberries and can be used for strawberry quality improvement.

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  • In Fragaria × ananassa, eugenol content was higher in 'Benihoppe' than 'Xiaobai', likely due to enhanced expression of FaEGS1a.

    “In contrast, the content of eugenol was higher in 'Benihoppe', likely due to enhanced expression of FaEGS1a, highlighting the genetic basis of somaclonal variation in volatile compound profiles and offering potential for improving strawberry quality through genetic manipulation [134].”

    From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [134]

  • In Fragaria × ananassa, mutant 'Xiaobai' had elevated levels of volatile compounds ethyl isovalerate, ethyl hexanoate, and linalool compared to parent 'Benihoppe', correlated with increased expression of FaLOX6, FaHPL, FaADH, FaAAT, FaAAT1, FaDXS, FaMCS, and FaHDR.

    “The results revealed elevated levels of volatile compounds such as ethyl isovalerate, ethyl hexanoate, and linalool in 'Xiaobai', which correlated with signifi-cantly increased expression of genes including FaLOX6, FaHPL, FaADH, FaAAT, FaAAT1, FaDXS, FaMCS, and FaHDR.”

    From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [134]

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Authors
Zhang, Z., Yu, S., Zhang, Z., Zhang, J., & Li, H.
Affiliations
  • Shenyang Agricultural University (CN)
  • Liaoning Key Laboratory of Strawberry Breeding and Cultivation, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
ORCID
Venue
Plants
Publisher
MDPI AG
Published
2023-03-01
Volume
12
Issue
5
Pages
1109
ISSN
2223-7747 (ISSN-L)
DOI
10.3390/plants12051109
PMC
PMC10005569
OpenAlex
W4322760652
Cited by
9
References
63
Citations by year
2025: 5 · 2024: 3 · 2023: 1
FWCI
1.41
Type
journal article
Language
en
Open access
Licence
Locations
  • Plants (publishedVersion, cc-by)
  • SSRN Electronic Journal (acceptedVersion)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (32130092, 2020-MS-205, 2020JH2/10200032, RC220306)
  • Natural Science Foundation of Liaoning Province (32130092, 2020-MS-205, 2020JH2/10200032, RC220306)
  • Liaoning Key R&amp;D Program (32130092, 2020-MS-205, 2020JH2/10200032, RC220306)
  • Shenyang Young and Middle-aged Science and Technology Innovation Talents Support Plan (32130092, 2020-MS-205, 2020JH2/10200032, RC220306)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Postharvest Quality and Shelf Life Management
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Berry genetics and cultivation research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Keywords
strawberry, somaclonal variation, fruit volatiles, ethyl esters, aroma compounds, eugenol, linalool, nerolidol

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