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Chemical Protein Crosslinking-Coupled Mass Spectrometry Reveals Interaction of LHCI with LHCII and LHCSR3 in Chlamydomonas reinhardtii

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Mosebach, Laura, Ozawa, Shin-Ichiro, Younas, Muhammad, Xue, Huidan, Scholz, Martin, Takahashi, Yuichiro, Hippler, Michael·2024·Plants
DOI 10.3390/plants13121632PMC PMC11207971

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Abstract

The photosystem I (PSI) of the green alga Chlamydomonas reinhardtii associates with 10 light-harvesting proteins (LHCIs) to form the PSI-LHCI complex. In the context of state transitions, two LHCII trimers bind to the PSAL, PSAH and PSAO side of PSI to produce the PSI-LHCI-LHCII complex. In this work, we took advantage of chemical crosslinking of proteins in conjunction with mass spectrometry to identify protein–protein interactions between the light-harvesting proteins of PSI and PSII. We detected crosslinks suggesting the binding of LHCBM proteins to the LHCA1-PSAG side of PSI as well as protein–protein interactions of LHCSR3 with LHCA5 and LHCA3. Our data indicate that the binding of LHCII to PSI is more versatile than anticipated and imply that LHCSR3 might be involved in the regulation of excitation energy transfer to the PSI core via LHCA5/LHCA3.

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Metadata

Authors
Mosebach, L., Ozawa, S.-I., Younas, M., Xue, H., Scholz, M., Takahashi, Y., & Hippler, M.
Affiliations
  • University of Münster (DE)
  • Okayama University (JP)
Corresponding author
Hippler, Michael
ORCID
Venue
Plants
Publisher
MDPI AG
Published
2024-06-13
Volume
13
Issue
12
Pages
1632
ISSN
2223-7747 (ISSN-L)
DOI
10.3390/plants13121632
PMC
PMC11207971
OpenAlex
W4399623903
Cited by
4
References
1
Citations by year
2026: 2 · 2025: 1 · 2024: 1
FWCI
0.54
Type
journal article
Language
en
Open access
Licence
Locations
  • Plants (publishedVersion, cc-by)
  • Preprints.org (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • DFG (Deutsche Forschungsgemeinschaft) (HI 739/13-1-3, G-1483-207/2018, 24K09488, 21H02510, 21K06217, 23H04959)
  • German-Israeli Foundation for Scientific Research and Development (HI 739/13-1-3, G-1483-207/2018, 24K09488, 21H02510, 21K06217, 23H04959)
  • JSPS KAKENHI (HI 739/13-1-3, G-1483-207/2018, 24K09488, 21H02510, 21K06217, 23H04959)
  • RECTOR program (Okayama University, Japan) (HI 739/13-1-3, G-1483-207/2018, 24K09488, 21H02510, 21K06217, 23H04959)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
  • Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
Keywords
light-harvesting complex II, LHCSR3, protein-protein interaction, Chlamydomonas reinhardtii, photosystem I, photosystem II, state transitions, excitation energy transfer, light-harvesting proteins, chemical crosslinking

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