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Cited by 7Cholesterol and Lipid MetabolismMetabolomics and Mass Spectrometry StudiesAnalytical Methods in Pharmaceuticals

Separation and Determination of Some of the Main Cholesterol-Related Compounds in Blood by Gas Chromatography-Mass Spectrometry (Selected Ion Monitoring Mode)

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Valverde-Som, Lucia, Carrasco-Pancorbo, Alegría, Sierra, Saleta, Santana, Soraya, Ruiz-Samblás, Cristina, Navas, Natalia, Burgos, Javier, Cuadros-Rodríguez, Luis·2018·Separations
DOI 10.3390/separations5010017
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Abstract

Oxysterols are metabolites produced in the first step of cholesterol metabolism, which is related to neurodegenerative disorder. They can be detected by testing blood, plasma, serum, or cerebrospinal fluid. In this study, some cholesterol precursors and oxysterols were determined by gas chromatography coupled to mass spectrometry. The selected cholesterol-related compounds were desmosterol, lathosterol, lanosterol, 7α-hydroxycholesterol, 7β-hydroxycholesterol, 24(S)-hydroxycholesterol, 25-hydroxycholesterol, 7-ketocholesterol, and 27-hydroxycholesterol. A powerful method was developed and validated considering various analytical parameters, such as linearity index, detection and quantification limits, selectivity and matrix effect, precision (repeatability), and trueness (recovery factor) for each cholesterol-related compound. 7α-hydroxycholesterol, 7β-hydroxycholesterol, and desmosterol exhibited the lowest detection and quantification limits, with 0.01 and 0.03 μg/mL, respectively, in the three cases. 7-ketocholesterol and lathosterol showed matrix effect percentages between 95.5% and 104.8%, respectively (demonstrating a negligible matrix effect), and very satisfactory repeatability values (i.e., overall performance of the method). Next, the method was applied to the analysis of a very interesting selection of mouse plasma samples (9 plasma extracts of non-transgenic and transgenic mice that had been fed different diets). Although the number of samples was limited, the current study led to some biologically relevant conclusions regarding brain cholesterol metabolism.

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  • Under the GC-MS analytical conditions, only cholesterol and desmosterol were detected and identified by comparison with authentic standards and published mass spectral data.

    “Under these analytical conditions, only cholesterol and desmosterol were detected and identified by comparison with authentic standards and published mass spectral data (Valverde-Som et al., 2018).”

    From Impaired astrocyte-to-neuron cholesterol trafficking drives synaptic dysfunction in Rett syndrome (2026) · cites this paper as Valverde-Som et al., 2018

    Section 3.3, “Application of the Method to the Analysis of Biological Samples” (page unavailable), reports several compounds besides cholesterol and desmosterol in mouse plasma, including 27-hydroxycholesterol and 24-hydroxycholesterol in every sample. The source therefore contradicts the claim that only those two compounds were detected under its analytical conditions. The cited passage also does not establish identification by comparison with published mass spectral data. (LLM, conf=0.91)

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Authors
Valverde-Som, L., Carrasco-Pancorbo, A., Sierra, S., Santana, S., Ruiz-Samblás, C., Navas, N., Burgos, J., & Cuadros-Rodríguez, L.
Affiliations
  • Department of Analytical Chemistry, Faculty of Science, University of Granada, C/Fuentenueva s/n, E-18071 Granada, Spain
  • Neuron Bio, P.T.S. Granada, C/Avicena 4, E-18016 Granada, Spain
  • Universidad de Granada (ES)
ORCID
Venue
Separations
Publisher
MDPI AG
Published
2018-03-05
Volume
5
Issue
1
Pages
17
ISSN
2297-8739 (ISSN-L)
DOI
10.3390/separations5010017
OpenAlex
W2789522230
Cited by
7
References
41
Citations by year
2025: 2 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 1
FWCI
0.52
Type
journal article
Language
en
Open access
Licence
Locations
  • Separations (publishedVersion, cc-by)
  • Institutional Repository of the University of Granada (University of Granada) (submittedVersion, cc-by)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Universidad de Granada
  • H2020 European Research Council
Topics
  • Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
  • Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
Keywords
oxysterols, cholesterol precursors, desmosterol, lathosterol, lanosterol, 7α-hydroxycholesterol, 7β-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 7-ketocholesterol, 27-hydroxycholesterol, plasma analysis, mouse plasma, transgenic mice, diet, brain cholesterol metabolism, neurodegenerative diseases, method validation

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