Performance and Mechanism of Neuroleukin in the Growth and Survival of Sertoli Cell-Induced Neurons in a Coculture System
Open accessAbstract
Sertoli cells (SCs), which are recognized as the "nurse cells" of the testis due to their important biofunctions, have been used in cotransplantation with neurons in cell therapy. However, it is not clear whether SCs influence neuronal communication and survival. In this study, we showed that approximately 60% of cortical neural stem cells (NSCs) cocultured with SCs differentiated into mature neurons. In addition, the neurite outgrowth and neuronal survival rates were significantly enhanced in the coculture system compared with differentiated neurons induced by a differentiation medium. The neuroleukin (NLK) secretion of SCs was also identified at the RNA and protein level, and the roles of NLK in neuromorphology and physiological regulation were systematically investigated for the first time. These results not only highlight the significance of paracrine regulation of NSCs by SCs but also confirm the role NLK plays in the differentiation and survival of NSCs. Finally, we proposed a possible hypothesis for the mechanism of NLK in the growth and survival of SC-induced neurons based on Western blotting results, which is that NLK secreted by SCs activates the Ras/Raf/MEK/Erk, Jak/Stat, and PI3K/Akt pathways, but not the NF-κB pathway, in neurons resulting in their growth and survival.
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NLK may support survival of sensory and spinal neurons and stimulate axonal growth.
“may support survival of sensory and spinal neurons and stimulate axonal growth [10–13]”
From Cerebrospinal fluid levels of the neurotrophic factor neuroleukin are increased in early Alzheimer’s disease, but not in cerebral amyloid angiopathy (2021) · cites this paper as [11]
In the extracellular space, NLK supports neuronal survival and stimulates axonal growth.
“In the extracellular space NLK supports neuronal survival and stimulates axonal growth [10–13]”
From Cerebrospinal fluid levels of the neurotrophic factor neuroleukin are increased in early Alzheimer’s disease, but not in cerebral amyloid angiopathy (2021) · cites this paper as [11]
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References 42 · Cited by 14
- Authors
- Deng, L., Shi, B., Zhuang, Y., Chu, J., Shi, X., Zhang, S., & Guo, M.
- Affiliations
- East China University of Science and Technology (CN)
- The University of Adelaide (AU)
- Capital Medical University (CN)
- Corresponding author
- Shi, Bingyang
- Shi, Xiaolin
- Guo, Meijin
- Venue
- Cell Transplantation
- Publisher
- SAGE Publications
- Published
- 2014-04
- Volume
- 23
- Issue
- 3
- Pages
- 381-394
- ISSN
- 0963-6897 (ISSN-L)
- 1555-3892
- OpenAlex
- W2332285656
- Cited by
- 14
- References
- 42
- Citations by year
- 2022: 1 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 6 · 2017: 1 · 2015: 1
- FWCI
- 0.12
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cell Transplantation (publishedVersion)
- PubMed (publishedVersion)
- Adelaide Research & Scholarship (AR&S) (University of Adelaide) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- neuroleukin, Sertoli cells, neuronal differentiation, neuronal survival, co-culture, paracrine signaling, neurite outgrowth, RAS/MAPK pathway, JAK/STAT signaling, PI3K/Akt signaling, NF-κB signaling, cell therapy, testis
- MeSH terms
- Amino Acid Sequence, Animals, Cells, Cultured, Glucose-6-Phosphate Isomerase, Male, Molecular Sequence Data, Neurons, Sertoli Cells, Cyclic AMP Response Element-Binding Protein, Coculture Techniques, MAP Kinase Kinase 1, Proto-Oncogene Proteins c-akt, Mice, Janus Kinase 1, Neurogenesis, Neural Stem Cells
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