Development of a novel mouse constipation model
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AIM: To establish a novel mouse constipation model. METHODS: Animals were randomly divided into three groups, and intragastrically administered 0-4 °C saline (ice-cold group) or 15-20 °C saline (saline control group) daily for 14 d, or were left untreated (blank control group). Stools were collected 3-24 h after treatment to record the wet and dry weights and the stool form. Intestinal propulsion experiments were carried out and defecation time was measured for six days continuously after suspending treatments. The expressions of PGP9.5 were detected by immunohistochemistry. RESULTS: Based on the percentage of stool weight changes compared with baseline (before irritation) in 9-14 d, stool weight changes were classified into three levels. Each level shows a different body state, which is state I (no change: plus or minus 5%), state II (slightly decreased: 5%-15%) and state III (decreased: 15%-25%). In state III, between day 9-14, the stool weights decreased by 15%-25% compared with the baseline, and changed at a rate > 10% compared with blank control values, and the stools became small and dry. Additionally, intestinal functions degenerated in these animals, and PGP9.5-positive expression markedly decreased in jejunum, ileum and proximal colon myenteric plexus. CONCLUSION: Irritation with ice-cold saline is a stable, repeatable method in building constipation model in mice for exploring the pathogenesis and treatment options of constipation, and the change of stool weight and size may serve as a useful tool to judge a constipation model success or not.
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From Autism Spectrum Disorder in Children Is Not Associated With Abnormal Autonomic Nervous System Function: Hypothesis and Theory (2022) · cites this paper as [16]
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References 27 · Cited by 31
- Authors
- Liang, C., Wang, K.-Y., Yu, Z., & Xu, B.
- Affiliations
- Nanjing University of Chinese Medicine (CN)
- Venue
- World Journal of Gastroenterology
- Publisher
- Baishideng Publishing Group Inc.
- Published
- 2016
- Volume
- 22
- Issue
- 9
- Pages
- 2799
- ISSN
- 1007-9327 (ISSN-L)
- PMC
- PMC4778002
- OpenAlex
- W2327954021
- Cited by
- 31
- References
- 27
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 3 · 2023: 4 · 2022: 4 · 2021: 3 · 2020: 4 · 2019: 2 · 2018: 1 · 2016: 1
- FWCI
- 1.46
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- World Journal of Gastroenterology (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (973 Program, 81202744, 81373749, 2011CB505206)
- National Science Council
- Nanjing University of Chinese Medicine
- Nanjing University
- National Key Research and Development Program of China (Program 973)
- Topics
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Health Sciences › Medicine › Surgery › Congenital gastrointestinal and neural anomalies
- Health Sciences › Medicine › Rheumatology › Pelvic floor disorders treatments
- Keywords
- constipation, mouse model, intestinal propulsion, stool weight, stool consistency, PGP 9.5, myenteric plexus, intestinal motility, defecation time
- MeSH terms
- Animals, Cold Temperature, Constipation, Defecation, Disease Models, Animal, Feces, Gastrointestinal Motility, Intestinal Mucosa, Intestines, Male, Mice, Inbred C57BL, Sodium Chloride, Time Factors, Ubiquitin Thiolesterase
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