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Cited by 21MicroRNA in disease regulationEpigenetics and DNA MethylationCancer-related molecular mechanisms research

hsa-mir-183 is frequently methylated and related to poor survival in human hepatocellular carcinoma

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Anwar, Sumadi Lukman, Krech, Till, Hasemeier, Britta, Schipper, Elisa, Schweitzer, Nora, Vogel, Arndt, Kreipe, Hans, Buurman, Reena +2 more·2017·World Journal of Gastroenterology
DOI 10.3748/wjg.v23.i9.1568PMC PMC5340808
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Abstract

AIM: To screen clinically relevant microRNAs (miRNAs) silenced by DNA methylation in human hepatocellular carcinoma (HCC). METHODS: Knockdown of DNA methyltransferases (DNMTs) using siRNAs and miRNA profiling in HCC cell lines were performed to identify DNA hypermethylation-mediated miRNA downregulation. Confirmation using individual quantitative real-time PCR (qRT-PCR) assays was then performed followed by DNA methylation quantification at the promoter of the miRNA genes. Quantification of DNA methylation and miRNA expression was then performed in primary HCC tumor samples and related with clinicopathological variables. RESULTS: is specific for the malignant transformation of hepatocytes. CONCLUSION: is a frequent event in HCC and potentially useful as a novel surrogate diagnostic and prognostic marker.

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  • In hepatocellular carcinoma, miR-23a/b are most probably derived from adipocytes and are upregulated.

    “miR-23a/b are most probably derived from adipocytes and are upregulated.”

    From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [72]

    The Results section ('Upregulated miRNAs after DNMT knockdown'; page not specified) reports that the hsa-miR-23 cluster was upregulated in HCC cell lines after DNMT knockdown. It does not identify miR-23a/b specifically, establish upregulation in HCC generally, or suggest an adipocyte origin. (LLM, conf=0.82)

    Experimental — accuracy not yet measured

  • miR-23a/b are transferred to hepatocellular carcinoma cancer cells via exosomes, promoting the growth and migration of HCC cells.

    “These miRNAs are then transferred to cancer cells via exosomes, thus promoting the growth and migration of HCC cells.”

    From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [72]

    The Results passage reports miR-23 upregulation after DNMT knockdown, while the Discussion concerns miR-183 methylation in HCC. Neither addresses exosomal transfer of miR-23a/b or its proposed effects on HCC-cell growth and migration; searches for exosomes and miR-23a/b growth and migration found no supporting passage. No page numbers were provided in our copy. (LLM, conf=0.76)

    Experimental — accuracy not yet measured

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Research works printed but not linked: 25

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Cited by

Sorted by citations

Authors
Anwar, S. L., Krech, T., Hasemeier, B., Schipper, E., Schweitzer, N., Vogel, A., Kreipe, H., Buurman, R., Skawran, B., & Lehmann, U.
Affiliations
Universitas Gadjah Mada (ID)
Corresponding author
Lehmann, Ulrich
ORCID
Venue
World Journal of Gastroenterology
Publisher
Baishideng Publishing Group Inc.
Published
2017
Volume
23
Issue
9
Pages
1568
ISSN
1007-9327 (ISSN-L)
DOI
10.3748/wjg.v23.i9.1568
PMC
PMC5340808
OpenAlex
W2591904920
Cited by
21
References
0
Citations by year
2025: 2 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 8 · 2018: 2 · 2017: 1
FWCI
1.29
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • World Journal of Gastroenterology (publishedVersion, cc-by-nc)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
  • Deutsche Forschungsgemeinschaft (SFB TRR77, TRR77)
  • Medizinischen Hochschule Hannover
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
Keywords
DNA methylation, hepatocellular carcinoma, microRNA silencing, DNA methyltransferases, miRNA expression profiling, prognostic factors, survival, promoter methylation
MeSH terms
Aged, Female, Carcinoma, Hepatocellular, Humans, Liver, Liver Neoplasms, Male, Middle Aged, Prognosis, Sulfites, Biomarkers, Tumor, Treatment Outcome, CpG Islands, DNA Methylation, Hepatocytes, RNA, Small Interfering, MicroRNAs, Epigenesis, Genetic, Cell Line, Tumor, Hep G2 Cells, Real-Time Polymerase Chain Reaction

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