Beta-glucan induced trained immunity is associated with changes in gut Nccrp-1+ and Mpeg-1+cell populations in rag1-/- zebrafish
Open accessAbstract
This study determined the effects of beta-glucan and Resiquimod (R848) on the non-specific cytotoxic cell (NCC), Natural Killer cell (NK) and macrophage/monocyte cell populations in the gut of rag1 -/-zebrafish.This study also determined if these changes were long-term and if changes in cell populations were associated with increased survival.Innate immune cells in rag1 -/-zebrafish include a decreased lymphocyte-like cell population with NCC's and NK cells, a macrophage/monocyte population, and neutrophils.Rag1 -/- zebrafish were exposed to phosphate buffered saline (PBS), beta-glucan or R848 by intracoelomic injection and after four weeks, gut samples were collected to analyze cell type distribution and protein expression of Nccrp-1 (NCC cells), Mpeg-1 (macrophages/monocytes) and Nitr9 (NK cells) by flow cytometry and western blot analyses.A separate group of rag1 -/-zebrafish was gavage challenged with Edwardsiella ictaluri at 4 weeks post PBS, beta-glucan or R848 injection and survival and cell type distribution were analyzed.There were significantly more NCCs and macrophages in rag1 -/-zebrafish gut tissue 4 weeks post beta-glucan injection when compared to PBS injected control tissues.There were significant differences in the relative protein expression of Mpeg-1 and Nitr9 after stimulation with beta-glucan or R848.Following gavage bacterial challenge, rag1 -/-zebrafish previously exposed to beta-glucan had significantly higher survival than rag1 -/- zebrafish previously exposed to PBS and significantly more NCCs and macrophages in the gut.These findings demonstrate that resident gut NCCs and macrophages in rag1 -/-zebrafish increase in number and undergo immune-modulation or training and contribute to increased survival after exposure to an enteric pathogen four weeks post immune-modulation.
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Long-term protection one month after oral bacterial exposure was demonstrated using the rag1−/− model.
“Further use of the rag1−/− model demonstrated long-term protection one month after oral bacterial exposure [3].”
From Trained Immunity Provides Long-Term Protection against Bacterial Infections in Channel Catfish (2022) · cites this paper as [3]
The Survival section says rag1−/− zebrafish were orally challenged with bacteria one month after beta-glucan or control treatment, then monitored for deaths for 15 days. The Discussion reports significantly higher survival in beta-glucan-treated fish. Thus the paper supports protection against an oral bacterial challenge, but not protection demonstrated one month after bacterial exposure; the one-month interval preceded the challenge. (LLM, conf=0.89)
Experimental — accuracy not yet measuredTeleost innate immune cells were shown to mediate protective immunity in T and B cell deficient rag1−/− zebrafish.
“Further, teleost innate immune cells were shown to mediate protective immunity in the T and B cell deficient rag1−/− zebrafish [2,3].”
From Trained Immunity Provides Long-Term Protection against Bacterial Infections in Channel Catfish (2022) · cites this paper as [3]
The Survival results (page unavailable) show that beta-glucan-treated rag1−/− zebrafish survived bacterial challenge significantly more often than controls. The Discussion (page unavailable) associates that protection with increased innate immune cell populations, but does not establish that those cells mediated the protection; that causal wording overstates the finding. (LLM, conf=0.82)
Experimental — accuracy not yet measuredBeta glucan induced trained immunity associated with macrophage populations in rag1−/− zebrafish.
“Beta glucan induced trained immunity associated with macrophage populations in rag1−/− zebrafish [3].”
From Trained Immunity Provides Long-Term Protection against Bacterial Infections in Channel Catfish (2022) · cites this paper as [3]
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Cites
Characterization of rag1 mutant zebrafish leukocytes
Petrie-Hanson, L., Hohn, C., & Hanson, L. (2009) · BMC Immunology · doi:10.1186/1471-2172-10-8 · Cited by 77 · see citing works
Self-citationRag1−/− Mutant Zebrafish Demonstrate Specific Protection following Bacterial Re-Exposure
Hohn, C., & Petrie-Hanson, L. (2012) · PLoS ONE · doi:10.1371/journal.pone.0044451 · Cited by 57 · see citing works
Self-citationMuire, P. J., Hanson, L. A., Wills, R., & Petrie-Hanson, L. (2017) · PLOS ONE · doi:10.1371/journal.pone.0184077 · Cited by 21 · see citing works
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Cited by
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Trained Immunity Provides Long-Term Protection against Bacterial Infections in Channel Catfish
Petrie-Hanson, L., & Peterman, A. E. (. (2022) · Pathogens · doi:10.3390/pathogens11101140 · Cited by 19 · see citing works
Self-citation
- Authors
- Peterman, B., & Petrie-Hanson, L.
- Affiliations
- Mississippi State University (US)
- Venue
- Journal of Aquaculture, Marine Biology & Ecology
- Publisher
- Kosmos Publishers
- Published
- 2021
- Volume
- 2021
- Issue
- 01
- ISSN
- 2692-1529 (ISSN-L)
- OpenAlex
- W3197526706
- Cited by
- 4
- References
- 3
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 1 · 2021: 1
- FWCI
- 0.28
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Journal of Aquaculture Marine Biology & Ecology (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune responses and vaccinations
- Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- trained immunity, β-glucan, resiquimod, natural killer cells, macrophages, monocytes, MPEG-1, Edwardsiella ictaluri, survival, immunomodulation, innate immunity
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