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The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

Luhr, Morten, Szalai, Paula, Engedal, Nikolai·2018·Journal of Visualized Experiments
DOI 10.3791/57971
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Research works printed but not linked: 37

  1. Rubinsztein, D. C., & Frake, R. A. Yoshinori Ohsumi's Nobel Prize for mechanisms of autophagy: from basic yeast biology to therapeutic potential. J R Coll Physicians Edinb. 46 (4), 228-233, (2016). · DOI
  2. Mizushima, N. The exponential growth of autophagy-related research: from the humble yeast to the Nobel Prize. FEBS Lett. 591 (5), 681-689, (2017). · DOI
  3. Seglen, P. O. et al. Macroautophagic cargo sequestration assays. Methods. 75 25-36, (2015). · DOI
  4. Høyvik, H., Gordon, P. B., & Seglen, P. O. Use of a hydrolysable probe, [14C]lactose, to distinguish between pre-lysosomal and lysosomal steps in the autophagic pathway. Exp Cell Res. 166 (1), 1-14, (1986). · DOI
  5. Plomp, P. J., Gordon, P. B., Meijer, A. J., Hoyvik, H., & Seglen, P. O. Energy dependence of different steps in the autophagic-lysosomal pathway. J Biol Chem. 264 (12), 6699-6704, (1989). · DOI
  6. Holen, I., Gordon, P. B., & Seglen, P. O. Protein kinase-dependent effects of okadaic acid on hepatocytic autophagy and cytoskeletal integrity. Biochem J. 284 (Pt 3) 633-636, (1992). · DOI
  7. Kabeya, Y. et al. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. Embo j. 19 (21), 5720-5728, (2000). · DOI · Paper page
  8. Szalai, P. et al. Autophagic bulk sequestration of cytosolic cargo is independent of LC3, but requires GABARAPs. Exp Cell Res. 333 (1), 21-38, (2015). · DOI · Paper page
  9. Nguyen, T. N. et al. Atg8 family LC3/GABARAP proteins are crucial for autophagosome-lysosome fusion but not autophagosome formation during PINK1/Parkin mitophagy and starvation. J Cell Biol. 215 (6), 857-874, (2016). · DOI · Paper page
  10. Pontano Vaites, L., Paulo, J. A., Huttlin, E. L., & Harper, J. W. Systematic analysis of human cells lacking ATG8 proteins uncovers roles for GABARAPs and the CCZ1/MON1 regulator C18orf8/RMC1 in macro and selective autophagic flux. Mol Cell Biol. 10.1128/mcb.00392-17, (2017). · DOI · Paper page
  11. Kopitz, J., Kisen, G. O., Gordon, P. B., Bohley, P., & Seglen, P. O. Nonselective autophagy of cytosolic enzymes by isolated rat hepatocytes. J Cell Biol. 111 (3), 941-953, (1990). · DOI
  12. Bowman, E. J., Siebers, A., & Altendorf, K. Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells. Proc Natl Acad Sci U S A. 85 (21), 7972-7976, (1988). · DOI
  13. Hadjivassiliou, A. G., & Rieder, S. V. The enzymatic assay of pyruvic and lactic acids. A definitive procedure. Clin Chim Acta. 19 (3), 357-361, (1968). · DOI
  14. Bergmeyer, H. U., & Bernt, E. in Methods of enzymatic analysis (2nd English ed.). Vol. 2 (ed H.U. Bergmeyer) 574-579 Verlag Chemie, (1974).
  15. Luhr, M. et al. A Simple Cargo Sequestration Assay for Quantitative Measurement of Nonselective Autophagy in Cultured Cells. Methods Enzymol. 587 351-364, (2017). · DOI
  16. Mousavi, S. A. et al. Effects of inhibitors of the vacuolar proton pump on hepatic heterophagy and autophagy. Biochim Biophys Acta. 1510 (1-2), 243-257, (2001). · DOI
  17. Seglen, P. O., & Gordon, P. B. 3-Methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes. Proc Natl Acad Sci U S A. 79 (6), 1889-1892, (1982). · DOI
  18. Sætre, F., Hagen, L. K., Engedal, N., & Seglen, P. O. Novel steps in the autophagic-lysosomal pathway. Febs j. 282 (11), 2202-2214, (2015). · DOI · Paper page
  19. Seglen, P. O., Øverbye, A., & Sætre, F. Sequestration assays for mammalian autophagy. Methods Enzymol. 452 63-83, (2009). · DOI · Paper page
  20. Hurley, J. H., & Young, L. N. Mechanisms of Autophagy Initiation. Annu Rev Biochem. 86 225-244, (2017). · DOI
  21. Thastrup, O., Cullen, P. J., Drobak, B. K., Hanley, M. R., & Dawson, A. P. Thapsigargin, a tumor promoter, discharges intracellular Ca2+ stores by specific inhibition of the endoplasmic reticulum Ca2(+)-ATPase. Proc Natl Acad Sci U S A. 87 (7), 2466-2470, (1990). · DOI
  22. Kuma, A. et al. The role of autophagy during the early neonatal starvation period. Nature. 432 (7020), 1032-1036, (2004). · DOI · Paper page
  23. Komatsu, M. et al. Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J Cell Biol. 169 (3), 425-434, (2005). · DOI · Paper page
  24. Saitoh, T. et al. Atg9a controls dsDNA-driven dynamic translocation of STING and the innate immune response. Proc Natl Acad Sci U S A. 106 (49), 20842-20846, (2009). · DOI · Paper page
  25. Gordon, P. B., & Seglen, P. O. Autophagic sequestration of [14C]sucrose, introduced into rat hepatocytes by reversible electro-permeabilization. Exp Cell Res. 142 (1), 1-14, (1982). · DOI
  26. Velikkakath, A. K., Nishimura, T., Oita, E., Ishihara, N., & Mizushima, N. Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets. Mol Biol Cell. 23 (5), 896-909, (2012). · DOI
  27. Øverbye, A., Sætre, F., Hagen, L. K., Johansen, H. T., & Seglen, P. O. Autophagic activity measured in whole rat hepatocytes as the accumulation of a novel BHMT fragment (p10), generated in amphisomes by the asparaginyl proteinase, legumain. Autophagy. 7 (9), 1011-1027, (2011). · DOI · Paper page
  28. Kominami, E., Hashida, S., Khairallah, E. A., & Katunuma, N. Sequestration of cytoplasmic enzymes in an autophagic vacuole-lysosomal system induced by injection of leupeptin. J Biol Chem. 258 (10), 6093-6100, (1983). · DOI
  29. Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., & Miyawaki, A. A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem Biol. 18 (8), 1042-1052, (2011). · DOI · Paper page
  30. Ogier-Denis, E. et al. A heterotrimeric Gi3-protein controls autophagic sequestration in the human colon cancer cell line HT-29. J Biol Chem. 270 (1), 13-16, (1995). · DOI
  31. Seglen, P. O., Gordon, P. B., Tolleshaug, H., & Høyvik, H. Use of [3H]raffinose as a specific probe of autophagic sequestration. Exp Cell Res. 162 (1), 273-277, (1986). · DOI
  32. Luhr, M., Sætre, F., & Engedal, N. The Long-lived Protein Degradation Assay: an Efficient Method for Quantitative Determination of the Autophagic Flux of Endogenous Proteins in Adherent Cell Lines. Bio-protocol. 8 (9), e2836, (2018). · DOI · Paper page
  33. Rønning, O. W., Pettersen, E. O., & Seglen, P. O. Protein synthesis and protein degradation through the cell cycle of human NHIK 3025 cells in vitro. Exp Cell Res. 123 (1), 63-72, (1979). · DOI
  34. Seglen, P. O., Grinde, B., & Solheim, A. E. Inhibition of the lysosomal pathway of protein degradation in isolated rat hepatocytes by ammonia, methylamine, chloroquine and leupeptin. Eur J Biochem. 95 (2), 215-225, (1979). · DOI
  35. Seglen, P. O., & Solheim, A. E. Valine uptake and incorporation into protein in isolated rat hepatocytes. Nature of the precursor pool for protein synthesis. Eur J Biochem. 85 (1), 15-25, (1978). · DOI
  36. Bauvy, C., Meijer, A. J., & Codogno, P. Assaying of autophagic protein degradation. Methods Enzymol. 452 47-61, (2009). · DOI
  37. Engedal, N., & Seglen, P. O. Autophagy of cytoplasmic bulk cargo does not require LC3. Autophagy. 12 (2), 1-3, (2016). · DOI · Paper page

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Metadata

Authors
Luhr, M., Szalai, P., & Engedal, N.
Venue
Journal of Visualized Experiments
Publisher
MyJove Corporation
Published
2018-07-27
Issue
137
ISSN
1940-087X
DOI
10.3791/57971
Cited by
7
References
7
Language
en

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