Anti-wrinkle effects of a tuna heart H2O fraction on Hs27 human fibroblasts
Open accessAbstract
With the increase in life expectancy, there is also growing interest in anti-aging treatments and technologies. The development of anti-aging functional drugs for the skin, and foods from natural sources, may offer solutions to this global matter. Aging involves structural, functional and biochemical changes that occur throughout cells and bodily tissues; the amount of hormones secreted from of all human organs, including the skin, decreases over time. Matrix metalloproteinase (MMP) genes (MMP-1 and -8) play an important role in the aging of skin fibroblasts. For example, an increased MMP expression causes accelerated aging and the degradation of skin elasticity-related genes. In the present study, we examined the anti-wrinkle effects of tuna heart extract which are mediated through the inhibition of MMPs in skin cells. Generally, tuna contains high concentrations of selenium and antioxidants, which serve to remove free radicals, and is known to delay skin and body aging. In addition, unsaturated fatty acids in tuna help to maintain the natural glossy look of skin, and increase skin elasticity, providing moisture for dry skin. A recent study confirmed the various bio-effects of boiled tuna extract and muscle. However, bioactivity studies using tuna heart are limited. Thus, in the present study, we obtained extracts and fractions of tuna heart, and examined their effects on Hs27 human fibroblast proliferation using an MTS assay. In addition, we measured procollagen type 1 levels and elastase activity, and performed β-galactosidase staining. We then measured the expression levels of phosphatidylinositol 3-kinase/Akt and MMP-related genes by western blot analysis and RT-PCR. Our results revealed that tuna heart extract decreased MMP expression by upregulating tissue inhibitors of metalloproteinase-1 (TIMP-1) and decreasing elastase activity, thus exerting anti-aging and anti-wrinkle effects by increasing collagen synthesis and promoting skin fibroblast proliferation. Thus, our data suggest that tuna heart (TH)-H2O fractions exert anti-wrinkle effects on Hs27 cells.
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A selenium-rich tuna heart extract enhanced collagen synthesis and promoted the proliferation of skin fibroblasts, thus having anti-ageing and anti-wrinkle effects.
“Kim et al. showed that a selenium-rich tuna heart extract enhanced collagen synthesis and promoted the proliferation of skin fibroblasts, thus having anti-ageing and anti-wrinkle effects [93].”
From Bioactive Compounds for Skin Health: A Review (2021) · cites this paper as [93]
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References 38 · Cited by 26
- Authors
- KIM, Y.-M., JUNG, H.-J., CHOI, J.-S., & NAM, T.-J.
- Affiliations
- Pukyong National University (KR)
- Corresponding author
- NAM, TAEK-JEONG
- Venue
- International Journal of Molecular Medicine
- Publisher
- Spandidos Publications
- Published
- 2015-11-11
- Volume
- 37
- Issue
- 1
- Pages
- 92-98
- ISSN
- 1107-3756 (ISSN-L)
- 1791-244X
- PMC
- PMC4687440
- OpenAlex
- W2096414791
- Cited by
- 26
- References
- 38
- Citations by year
- 2025: 5 · 2024: 1 · 2023: 3 · 2022: 6 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 3 · 2017: 1 · 2016: 1
- FWCI
- 1.87
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- International Journal of Molecular Medicine (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- National Research Foundation
- Ministry of Oceans and Fisheries (20130279)
- National Research Foundation of Korea
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
- Keywords
- anti-wrinkle effect, skin aging, matrix metalloproteinase inhibition, MMP-1, MMP-8, TIMP-1, elastase activity, collagen synthesis, fibroblast proliferation, phosphatidylinositol 3-kinase/Akt signaling, water fraction
- MeSH terms
- Animals, beta-Galactosidase, Biological Products, Cell Line, Fibroblasts, Gene Expression Regulation, Humans, Myocardium, Pancreatic Elastase, RNA, Messenger, Tuna, Water, Skin Aging, Phosphatidylinositol 3-Kinases, Matrix Metalloproteinases, Cell Proliferation, Proto-Oncogene Proteins c-akt
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