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Cited by 495Mesenchymal stem cell researchPluripotent Stem Cells ResearchHematopoietic Stem Cell Transplantation

Comparison of molecular profiles of human mesenchymal stem cells derived from bone marrow, umbilical cord blood, placenta and adipose tissue

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HEO, JUNE SEOK, CHOI, YOUJEONG, KIM, HAN-SOO, KIM, HYUN OK·2016·International Journal of Molecular Medicine
DOI 10.3892/ijmm.2015.2413PMC PMC4687432
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Abstract

Mesenchymal stem cells (MSCs) are clinically useful due to their capacity for self-renewal, their immunomodulatory properties and tissue regenerative potential. These cells can be isolated from various tissues and exhibit different potential for clinical applications according to their origin, and thus comparative studies on MSCs from different tissues are essential. In this study, we investigated the immunophenotype, proliferative potential, multilineage differentiation and immunomodulatory capacity of MSCs derived from different tissue sources, namely bone marrow, adipose tissue, the placenta and umbilical cord blood. The gene expression profiles of stemness-related genes [octamer-binding transcription factor 4 (OCT4), sex determining region Y-box (SOX)2, MYC, Krüppel-like factor 4 (KLF4), NANOG, LIN28 and REX1] and lineage‑related and differentiation stage-related genes [B4GALNT1 (GM2/GS2 synthase), inhibin, beta A (INHBA), distal-less homeobox 5 (DLX5), runt-related transcription factor 2 (RUNX2), proliferator‑activated receptor gamma (PPARG), CCAAT/enhancer-binding protein alpha (C/EBPA), bone morphogenetic protein 7 (BMP7) and SOX9] were compared using RT-PCR. No significant differences in growth rate, colony-forming efficiency and immunophenotype were observed. Our results demonstrated that MSCs derived from bone marrow and adipose tissue shared not only in vitro tri-lineage differentiation potential, but also gene expression profiles. While there was considerable inter-donor variation in DLX5 expression between MSCs derived from different tissues, its expression appears to be associated with the osteogenic potential of MSCs. Bone marrow-derived MSCs (BM-MSCs) significantly inhibited allogeneic T cell proliferation possibly via the high levels of the immunosuppressive cytokines, IL10 and TGFB1. Although MSCs derived from different tissues and fibroblasts share many characteristics, some of the marker genes, such as B4GALNT1 and DLX5 may be useful for the characterization of MSCs derived from different tissue sources. Collectively, our results suggest that, based on their tri-lineage differentiation potential and immunomodulatory effects, BM-MSCs and adipose tissue-derived MSCs (A-MSCs) represent the optimal stem cell source for tissue engineering and regenerative medicine.

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  • Other studies propose that ASCs have equal or superior osteogenic potential compared to BM-MSCs.

    “Some suggest that BM-MSCs exhibit greater osteogenic capacity [58,59,60,61,62], while others propose that ASCs have equal or superior potential [58,63,64,65,66].”

    From Mesenchymal Stem/Stromal Cells: Immunomodulatory and Bone Regeneration Potential after Tumor Excision in Osteosarcoma Patients (2023) · cites this paper as [66]

    The Results (Immunophenotype and differentiation potential; page not available) report osteogenic differentiation in both adipose-derived MSCs and bone-marrow MSCs. The Results (DLX5 expression and osteogenic potential; page not available) also found an osteogenic phenotype in both cell types across three donors, but do not establish that the adipose-derived cells had equal or superior osteogenic capacity. (LLM, conf=0.82)

    Experimental — accuracy not yet measured

  • Studies comparing the osteogenic potential of human BM-MSCs and ASCs have yielded mixed results.

    “Regarding bone tissue engineering, studies comparing the osteogenic potential of human BM-MSCs and ASCs have yielded mixed results.”

    From Mesenchymal Stem/Stromal Cells: Immunomodulatory and Bone Regeneration Potential after Tumor Excision in Osteosarcoma Patients (2023) · cites this paper as [66]

    The Results (Immunophenotype and differentiation potential; page unavailable) compare BM-MSCs with adipose-derived MSCs and find osteogenic differentiation in both. This supports the paper being an example of such a comparison, but our copy does not establish that studies comparing the two have yielded mixed results. (LLM, conf=0.83)

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Cited by

Sorted by citations

Authors
HEO, J. S., CHOI, Y., KIM, H.-S., & KIM, H. O.
Affiliations
Severance Hospital (KR)
Corresponding author
  • KIM, HAN-SOO
  • KIM, HYUN OK
ORCID
Venue
International Journal of Molecular Medicine
Publisher
Spandidos Publications
Published
2015-11-19
Volume
37
Issue
1
Pages
115-125
ISSN
  • 1107-3756 (ISSN-L)
  • 1791-244X
DOI
10.3892/ijmm.2015.2413
PMC
PMC4687432
OpenAlex
W2181115262
Cited by
495
References
1
Citations by year
2026: 35 · 2025: 33 · 2024: 69 · 2023: 61 · 2022: 69 · 2021: 75 · 2020: 56 · 2019: 31 · 2018: 27 · 2017: 30 · 2016: 9
FWCI
12.32
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • International Journal of Molecular Medicine (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • YUHSpace (Yonsei University Medical Library) (submittedVersion, cc-by-nc-nd)
Topics
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
Keywords
mesenchymal stem cells, bone marrow-derived stem cells, adipose-derived stem cells, placenta-derived stem cells, umbilical cord blood stem cells, multilineage differentiation, immunomodulation, allogeneic T cell proliferation, IL-10, TGF-β1, stemness gene expression, Dlx5, B4GALNT1, osteogenic differentiation
MeSH terms
Kruppel-Like Factor 4, Adipose Tissue, Adult, Bone Marrow Cells, Cells, Cultured, Female, Fetal Blood, Humans, Male, Osteogenesis, Placenta, Pregnancy, Transcription Factors, Immunophenotyping, Homeodomain Proteins, Cell Proliferation, Young Adult, Transcriptome, Mesenchymal Stem Cells

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